Kits and methods for determining total cholesterol

Abstract

The present invention relates to kits and methods for determining total cholesterol. The test kit comprises reagent group 1 and reagent group 2, and described reagent group 1 comprises chromogen and cholesterol esterase, and described reagent group 2 comprises 4-aminoantipyrine, peroxidase and cholesterol oxidase, wherein , the chromogen is or comprises N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and method of the present invention can reduce the interference caused by calcium dobesilate and / or etamsulfone present in the sample in the detection of total cholesterol.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting total cholesterol. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol exists in lipoproteins in the blood, and its existing forms include high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. Cholesterol is one of the indispensable important substances in animal tissue cells. It not only participates in the formation of cell membranes, but also is an important raw material for the synthesis of adrenal cortex hormones, bile acids, vitamin D and other physiologically active substances, and is also the main component of cell m...

AI Technical Summary

Problems solved by technology

[0015] The interference of calcium dobesilate and phenetamine on the enzymatic measurement of total cholesterol is a major inconvenience to patients who are administered these two drugs

For example, for diabetic patients, they are often accompanied by coronary heart disease, vascular arteriosclerosis, etc., and the interference of the administered calcium dobesilate on the enzymatic detection of total cholesterol may cause the total cholesterol level of diabetic patients to be incorrectly evaluated, covering up and delay illness

For patients undergoing surgery, the interference of the administered ethanol on the enzymatic detection of total cholesterol may make it impossible to correctly assess their total cholesterol levels, resulting in postoperative complications and affecting the prognosis of patients

At present, the enzymatic detection kits sold on the market generally have the defect that they cannot effectively resist the interference of calcium dobesilate and phensulfonate

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