Spreading oil suspension, and application thereof in control of insect diseases of crops
An oil suspending agent and film spreading technology, which is applied in the fields of application, botanical equipment and methods, preservation of human or animal body, etc. Good results with good spreadability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0023] In the present invention, the preparation method of described Beauveria bassiana comprises the following steps:
[0024] 1) Beauveria bassiana strain activation;
[0025] 2) inoculating the activated Beauveria bassiana into the Beauveria bassiana liquid culture medium for liquid culture, and stopping the cultivation for 30-50 hours to obtain the Beauveria bassiana culture solution;
[0026] 3) inoculating the culture solution of Beauveria bassiana obtained in the step 2) into the solid culture medium of Beauveria bassiana, and performing solid fermentation to obtain Beauveria bassiana.
[0027] The activation of the Beauveria bassiana strain in the present invention is to inoculate the preserved Beauveria bassiana strain into an eggplant-shaped bottle equipped with a nutrient agar medium, and cultivate for 8-12 hours at 25-28° C. for activation culture.
[0028] In the present invention, the inoculation amount of the activated Beauveria bassiana inoculated in the liqui...
Embodiment 1
[0049] The preparation of Beauveria bassiana: inoculate the preserved Beauveria bassiana bacterial classification in the eggplant type bottle that nutrient agar medium is housed, cultivate 9h at 26 ℃ and carry out activation culture; Beauveria bassiana after activation culture is with 25% of the inoculum was inoculated in the liquid medium after sterilized at 121°C for 35 minutes, and the liquid culture was carried out in the fermenter. The specific culture conditions were as follows: the temperature was 28°C; the pH value was 6.9; The ventilation rate of the culture is 1V / V min; the culture is stopped after 40 hours of cultivation, and the culture solution of Beauveria bassiana is obtained; the obtained culture solution of Beauveria bassiana is inoculated in a solid medium with an inoculum of 25%, and cultured on a solid Add distilled water to the base to make the water content of the solid medium 50%, and then carry out solid fermentation. The fermentation conditions are: the...
Embodiment 2
[0051] In percent by weight, 30% of Beauveria bassiana prepared in Example 1, 7.5% of nonylphenoxyethylene ether, 7.5% of methyl oleate, 2% of dodecyl alcohol ester, 8% of stearyl alcohol, propylene glycol Phenyl ether 7%, butyl ether acetate 1%, mineral spirits to make up to 100%.
[0052] Preparation process: In a mixing kettle with agitator, firstly, Beauveria bassiana, nonylphenoxyethylene ether, methyl oleate, dodecyl alcohol ester, stearyl alcohol, propylene glycol phenyl ether, butyl ether acetate , solvent oil, fully stirred and mixed, then pumped into a sand mill for sanding, and after sanding to a particle size of D90≤5 μm, the film-forming oil suspension of Beauveria bassiana containing spores can be obtained.
[0053] Following the same preparation process as in Example 2, the following examples were prepared.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Spread area | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More