Anti-H3N2 subtype canine influenza virus nucleoprotein monoclonal antibody hybridoma 2D10, and monoclonal antibody
A technology of H3N2-CIV-NP-2D10, monoclonal antibody, applied in the field of immunology, can solve problems such as influenza pandemic, achieve the effect of high specificity and sensitivity, and high secretion yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0019] The present invention will be further described in combination with specific experiments, but it is not limited to this.
[0020] Establishment of H3N2-CIV-NP-2D10 hybridoma cell line and preparation and identification of NP monoclonal antibodies against H3N2 subtype canine influenza virus
[0021] 1. Mouse Immunization
[0022] The H3N2 subtype canine influenza virus was inactivated and mixed with an equal volume of Freund's complete adjuvant, and then injected into the back of BALB / C mice subcutaneously at 50μg / 500μl each (Experimental Animal Center, Southern Medical University) , 6-8 weeks old females, 3) for the first immunization; after 2 weeks, the adjuvant was replaced with Freund’s incomplete adjuvant for the second routine immunization, the immunization method and dosage were the same as the first immunization; a small amount was collected after three routine immunizations Tail vein blood, indirect ELISA to detect serum titer. The results are shown in Table 1.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap