Supercharge Your Innovation With Domain-Expert AI Agents!

Vitamin C individualized quantitative compensating detecting method based on genetic typing

A detection method and genotyping technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem that the demand for vitamin C varies from person to person.

Inactive Publication Date: 2017-02-08
神州亿昊基因技术(北京)有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By detecting 4 gene polymorphisms related to vitamin C absorption, transport, and function, we can understand the efficiency level of metabolism and utilization of vitamin C in different individuals, so as to obtain a "tailor-made" vitamin C supplement plan for each person to solve different constitutions The demand for vitamin C varies from person to person and from person to person

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitamin C individualized quantitative compensating detecting method based on genetic typing
  • Vitamin C individualized quantitative compensating detecting method based on genetic typing
  • Vitamin C individualized quantitative compensating detecting method based on genetic typing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Sample collection and DNA template preparation:

[0060] Proceed as follows:

[0061] 1) Use oral swabs to collect the oral epithelial cells of the person to be tested. The method is to insert the swab into the oral cavity so that the head of the swab fully contacts the mucous membranes on the inside of the cheek and the upper and lower gums, and rubs up and down with the force of brushing teeth, while rotating the swab. Let the swab head fully touch the oral mucosa, and repeat this action for 1 minute.

[0062] 2) Using the standard buccal swab DNA extraction kit and corresponding steps, place the swab stained with buccal cells in 800 μL of normal saline, rinse for 20 seconds to make the cells fall off completely, stick to the wall of the centrifuge tube and squeeze dry the swab. For liquid, centrifuge at 12,000rpm for 5min.

[0063] 3) Discard 700 μL of supernatant, and the remaining 100 μL of supernatant, fully shake and mix for 15 seconds, add 200 μL of ...

Embodiment 2

[0069] Embodiment 2 primer design:

[0070] For the SLC23A1 gene and the NQO1 gene, specific amplification primers for common sites and rare sites were designed (the two primers differ only in terminal bases, denoted by F1 and F2, respectively), and corresponding reverse primers ( R indicates), 3 primers are combined to form a primer premix. The 5' ends of the two forward primers are respectively connected with different detection sequences for fluorescence detection. Specific amplification primers for common sites and rare sites are underlined, and detection primer sequences are indicated in lowercase fonts, where the part connected to F1 corresponds to blue fluorescence, and the part connected to F2 corresponds to red fluorescence. For GSTM1 gene and GSTT1 gene, the two primers in the master mix are F and R respectively, the specific sequence is underlined at the 3' end, and the detection sequence is indicated in lowercase font, corresponding to the above blue or red fluore...

Embodiment 3

[0085] The establishment of embodiment 3PCR detection system:

[0086] The PCR amplification system includes commercially purchased 2x Mix (including Taq enzyme, 4 kinds of dNTPs, and two probes corresponding to the detection primer sequence of the forward primer). The PCR system is as follows:

[0087] PCR amplification system (10μL):

[0088]

[0089] The PCR amplification procedure is as follows:

[0090]

[0091] Fluorescence reading:

[0092] In an environment below 40°C, read the fluorescence value.

[0093] Interpretation of results:

[0094] For the SLC23A1 gene, if the individual only contains G at the rs33972313 site, only the F1 primer can amplify, and the corresponding fluorescent signal is blue, and the site is recorded as GG; if the site only contains A, only the F2 primer can amplify Amplification, the corresponding fluorescent signal is red, and the site is recorded as AA; if the individual contains both G and A, both F1 and F2 primers can be amplifi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a vitamin C individualized quantitative compensating detecting method based on genetic typing. The vitamin C individualized quantitative compensating detecting method based on genetic typing comprises the following steps: step 1, sample collecting and DNA template preparing; step 2, primer designing, wherein specific amplification primers for a common site and a rare site are respectively designed, only basic groups at the tail ends of the two primers are different, and are respectively represented by F1 and F2, corresponding reverse primers are represented by R, and the three primers are combined to form primer premix liquor. Besides, ends 5' of two forward primers are respectively connected with different detecting sequences for fluorescence detection; and step 3, PCR detecting system establishing, wherein a fluorescence value is read under an environment with temperature lower than 40 DEG C, and results are read; and step 4, result analyzing and dose determining.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a genotype database of genes related to vitamin absorption, metabolism and late reaction, a genotyping method of vitamin-related genes, and a detection method for judging vitamin deficiency and excess. Background technique [0002] Vitamin C, also known as ascorbic acid, participates in important physiological and metabolic processes such as oxygen free radical scavenging, antibody formation, tissue repair, carbohydrate utilization, lipid and protein synthesis, etc. Long-term insufficient intake can lead to scurvy. In addition, vitamin C deficiency is significantly associated with the risk of diseases such as cardiovascular and cerebrovascular diseases, type 2 diabetes and tumors. Although the "Dietary Guidelines for Chinese Residents 2016" (hereinafter referred to as "Guidelines") gives the supplementary dosage of vitamin C relative to the Chinese population, each person's demand ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 魏晓星莫维克
Owner 神州亿昊基因技术(北京)有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com