Application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines for treating LPS-induced respiratory distress syndrome

A technology of protein simulation and respiratory distress, applied in drug combinations, respiratory diseases, peptide/protein components, etc., can solve the problem of no report on sepsis treatment

Inactive Publication Date: 2017-02-15
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Reverse-D-4F is a mimic peptide synthesized according to the reverse sequence of D-4F

Method used

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  • Application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines for treating LPS-induced respiratory distress syndrome
  • Application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines for treating LPS-induced respiratory distress syndrome
  • Application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines for treating LPS-induced respiratory distress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Reverse-D-4F treats LPS-induced lung injury model in rats.

[0020] Animal ARDS model was established, 24 male C57 mice were divided into 4 groups:

[0021] (1) Control group (intraperitoneal injection of PBS);

[0022] (2) Reverse-D-4F group (intraperitoneal injection of Reverse-D-4F 10mg / Kg)

[0023] (3) Model group (intraperitoneal injection of LPS 10mg / Kg);

[0024] (4) Reverse-D-4F treatment group (intraperitoneal injection of Reverse-D-4F 10mg / Kg and LPS10mg / Kg)

[0025] Two days after the model was made, the left lung was taken by intravenous injection of ketamine hydrochloride under general anesthesia, and the left lung was taken. The surface of the lung tissue was cleaned with absorbent paper. The weight of the left lung was considered as wet weight; the left lung was placed in a dry box at 60°C Dry for 48 hours, and then weigh the dry weight. The wet-dry weight ratio is used to evaluate the degree of pulmonary edema.

[0026] ELISA kit was used to detect the expression ...

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Abstract

The invention relates to an application of high-density protein mimic peptide Reverse-D-4F in preparation of medicines or kits for treating LPS-induced respiratory distress syndrome, the sequence of the high-density protein mimic peptide is Ac-FAEKFKEAVKDYFAKFWD-NH2. The Reverse-D-4F can inhibit pulmonary edema of an LPS-induced rat lung injury model, inhibit in-vivo inflammatory factors, alveolar wall thickening and intercellular space erythrocyte and leucocyte infiltration, as well as the generation of lung vascular endothelial inflammation markers (ICAM-1, VCAN-1 and iNOS) and the endothelial marks (eNOS and vWF) are increased. Reverse-D-4F promotes mobilization of EPCS of the LPS-induced rat lung injury model, no matter whether in vitro or in vivo, the Reverse-D-4F can be used for repairing the injured function of the EPCS of the LPS-induced rat lung injury model.

Description

Technical field [0001] The invention relates to the application field of high-density lipoprotein mimetic peptide Reverse-D-4F, in particular to the application in a medicine for treating LPS-induced respiratory distress syndrome. Background technique [0002] Acute Respiratory Distress Syndrome (ARDS) is a common clinical syndrome with a high mortality rate. The clinical manifestations are respiratory distress and difficulty. Hypoxia is corrected by traditional oxygen therapy. The pathogenesis of ARDS can be divided into biological and non-biological pathogens. Biological pathogens include bacteria, viruses, fungi, atypical pathogens and malignant tumors, while non-biological pathogens include acidic substances, drugs, toxic gas inhalation and mechanical ventilation related injuries. Initially, harmful substances such as endotoxin in the blood circulation damage pulmonary capillary endothelial cells (PCEC), resulting in increased endothelial cell permeability, as well as contra...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P11/00
CPCC07K7/08A61K38/00
Inventor 秦树存杨娜娜宋洪涛田华翟雷姚树桐
Owner TAISHAN MEDICAL UNIV
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