Method for preparing tricolor coding microsphere composition

A technology for encoding microspheres and compositions, applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of difficult multi-color decoding, complex detection equipment, and high cost in decoding and detection systems, and achieve the expansion of encoding capacity, The effect of improving sensitivity and accuracy, reducing manufacturing and application costs

Active Publication Date: 2017-02-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Traditional two-color fluorescent dye-based coded microspheres are loaded with organic fluorescent dyes inside the microspheres at the same time, and the fluorescence emission peak of the two-color fluorescein is relatively wide, causing interference between the two fluorescent substances, and the actual coding accuracy is limited.
[0005] 2. Multi-color (three-color or more) organic fluorescent dye-based coded microspheres have different optimal excitation spectra for each fluorescent molecule. In order to obtain ideal coding accuracy and high coding capacity, it is often necessary to use multi-color Laser excitation and detection equipment are complex and costly, which is not conducive to the miniaturization of equipment;
[0006] 3. Although multi-color quantum dot-encoded microspheres can use monochromatic laser to excite multi-color quantum dots, due to the large half-peak width of the fluorescence emission of quantum dots, the fluorescence spectra of quantum dots of different fluorescent colors interfere with each other. Cytometry decoding detection system is difficult to achieve high-precision multi-color decoding
[0007] 4. The existing host-guest structure coded microsphere coded fluorescent elements are coded by organic dyes or quantum dots. If monochromatic laser excitation is used for coding, there is also the problem that the color of fluorescent codes is limited.

Method used

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  • Method for preparing tricolor coding microsphere composition
  • Method for preparing tricolor coding microsphere composition
  • Method for preparing tricolor coding microsphere composition

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1 Preparation of the first type subball

[0041] In this embodiment, non-fluorescent mesoporous silica nanoparticles whose surface is modified with amino groups and whose particle size is 110 nm are used. As a modification of this specific implementation, the subballs can also be polymer particles; the particle size can also be 30-500nm; the surface of the subballs can also be modified with carboxyl groups or other functional groups.

Embodiment 2

[0042] Example 2 Preparation of the second type subball

[0043] In this specific embodiment, Europium with a central emission wavelength of 612nm (Eu 3+ ) Rare earth complex mesoporous silica particles are used as single-color sub-spheres. Mesoporous silica has a particle size of 110nm and is modified with amino groups (as a modification of this specific implementation, the surface of the sub-sphere can also be modified with functional groups such as hydroxyl, sulfhydryl, or carboxyl), and is selected for high quantum yield, stable fluorescence and available visible light Excited iridium metal ligand sensitizes Eu 3+ The typical preparation method is as follows:

[0044] Step 1: Ir(dfppy) 2 (pic-OH) preparation (see Biomaterials, 2014, 35(22): 5830-5839 for details)

[0045] First, 2-ethoxyethanol and water are mixed in a 250ml flask at a ratio of 3:1 (total volume is 120ml, reflux temperature is 120℃), and IrCl is added 3 ·3H 2 O (1 mmol, 355.3 mg) and 2-(2,4-difluorophenyl)pyridi...

Embodiment 3

[0053] Example 3 Preparation of the third type subball

[0054] In this specific embodiment, microspheres with fluorescent quantum dots with a central emission wavelength of 500 nm are assembled on the surface as monochromatic subspheres. The particle size of the microspheres is 110nm and is modified with amino groups (as a modification of this implementation, the surface of the subspheres can also be modified with functional groups such as hydroxyl groups); the quantum dots assembled on the surface of the subspheres choose CdSe@ZnS with a particle size of 1-10nm Nanocrystals (as a modification of this implementation, other nanocrystals with the same emission wavelength can also be used). The preparation method is the prior art and will not be detailed here.

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Abstract

The invention provides a method for preparing a tricolor coding microsphere composition. The method comprises the following steps: 1, preparing a sub-sphere; 2, preparing a mother sphere; and 3, assembling the sub-sphere and the mother sphere to form the microsphere composition, wherein at least three fluorescent substances are loaded on the microsphere composition, and at least one of the at least three fluorescent substances is a rare earth complex. The tricolor coding microsphere composition prepared through the method makes mono-color laser simultaneously excite tricolor fluorescence in order to carry out multicolor coding.

Description

Technical field [0001] The invention relates to a method for preparing a microsphere composition, in particular to a method for preparing a three-color coded microsphere composition. Background technique [0002] The existing multi-index detection system based on suspension array chips uses microspheres containing coded information to perform qualitative and quantitative analysis of proteins, nucleic acids, and cytokines at the same time, and is widely used in life science research, disease screening, drug screening and clinical diagnosis in. The core component of the suspension array technology is the microspheres with unique encoding information. In particular, the microspheres can be optically encoded by optical materials such as organic fluorescent dyes, Raman molecular tags, quantum dots, and polymer quantum dots. Rare earth refers to the lanthanide elements in the periodic table of chemical elements and the elements closely related to the lanthanide elements—yttrium and sc...

Claims

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Application Information

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IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/182G01N21/6486
Inventor 徐宏殷传唱古宏晨叶坚
Owner SHANGHAI JIAO TONG UNIV
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