Purpose of umbilical cord mesenchymal stem cell exosome

Abstract

The invention relates to the field of stem cell culture, and particularly relates to a purpose of umbilical cord mesenchymal stem cell exosome. Shown by experiments, with respect to culture experiments adopting a complete medium, by adopting co-culture of the umbilical cord mesenchymal stem cell exosome and peripheral blood stem cells, the amount of cells acquired by culture can be increased by about 1.5 times, and a clone formation amount is also increased by about 1.5 times. Through statistical analysis, compared with a culture effect without the umbilical cord mesenchymal stem cell exosome, the experiment effect with addition of the umbilical cord mesenchymal stem cell exosome has an extremely remarkable difference (p is smaller than 0.001).

Description

technical field

[0001] The invention relates to the field of stem cell culture, in particular to the use of umbilical cord mesenchymal stem cell exosomes. Background technique

[0002] Peripheral hematopoietic stem cells are hematopoietic stem cells present in peripheral blood. In 1979, Goldman et al. collected cryopreserved peripheral blood cells for a group of chronic myeloid leukemia patients in the accelerated phase or blast transition phase at the initial diagnosis, so that the patients returned to the chronic phase, and began the clinical application of peripheral blood hematopoietic stem cell transplantation (PBSCT).

[0003] Under normal circumstances, the content of stem / progenitor cells in peripheral blood accounts for 0.01% to 0.1% of mononuclear cells, which is equivalent to 1% to 10% of bone marrow. After mobilization, CD34 in peripheral blood MNCs + The number of cells and CFU were significantly higher than those of bone marrow. So far, it has been found that...

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