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Method for preparing peripheral nerve conduit by salt-induced phase transition

A peripheral nerve, nerve conduit technology, applied in the field of biomedical materials

Active Publication Date: 2017-02-22
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above two characteristics, the present invention provides a preparation method of a nerve guide with a single natural degradable polymer component and good mechanical strength. Excellent and single composition without adding any chemical substances such as cross-linking agents

Method used

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  • Method for preparing peripheral nerve conduit by salt-induced phase transition
  • Method for preparing peripheral nerve conduit by salt-induced phase transition
  • Method for preparing peripheral nerve conduit by salt-induced phase transition

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Using temperature-sensitive chitosan solution with a concentration of 0.4% as raw material, prepare a nerve conduit with an inner diameter of 0.5 mm and a length of 1 mm. After the solution forms a gel and has a certain strength, the mandrel is taken out together with the gel and placed in a 0.5% (w / v) NaCl solution to shrink for 12 hours. After the samples were taken out and pre-frozen at -20°C, they were freeze-dried for 12 hours. Remove mandrel 60 The temperature-sensitive chitosan nerve guide was prepared by Co irradiation sterilization.

Embodiment 2

[0034] Using temperature-sensitive chitosan solution with a concentration of 0.8% as raw material, prepare a nerve conduit with an inner diameter of 1 mm and a length of 10 mm. After the gel is formed and has a certain strength, the core rod is taken out together with the gel and placed in 2.5% (w / v) CH 3 COONH 4 Shrinkage in the solution for 48h. After the samples were taken out and pre-frozen at -30°C, they were freeze-dried for 18 hours. Remove mandrel 60 The temperature-sensitive chitosan nerve guide was prepared by Co irradiation sterilization.

Embodiment 3

[0036] Using temperature-sensitive chitosan solution with a concentration of 1% as raw material, prepare a nerve conduit with an inner diameter of 3mm and a length of 40mm. After the gel is formed and has a certain strength, the core rod is taken out together with the gel and placed in 5% (w / v) Na 2 HPO 4 and CH 3 Shrink in COONa solution for 24h. After the samples were taken out and pre-frozen at -40°C, they were freeze-dried for 24 hours. Remove the mandrel and sterilize it with 60Co radiation to form a temperature-sensitive chitosan nerve guide.

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Abstract

The invention discloses a method for preparing a peripheral nerve conduit by salt-induced phase transition. The peripheral nerve conduit is prepared from thermosensitive chitosan. thermosensitivity of the thermosensitive chitosan and properties of salt-induced phase transition are utilized, flowing gel is poured into a mold at a low temperature to shape by the aid of thermosensitivity of the thermosensitive chitosan, by utilizing the properties of the salt-induced phase transition, the shaped gel is immersed in a saline solution to enable phase transition so as to form an elastic gel body high in mechanical strength, then the elastic gel body is frozen and dried to shape, and finally the shaped gel body is subjected to <60>Co irradiation sterilization to form the peripheral nerve conduit. The prepared peripheral nerve conduit is high in mechanical strength, satisfies sewing requirements in clinic, is provided with abundant porous structures and is prone to interchange of nutrient substances. The method for preparing the peripheral nerve conduit by the salt-induced phase transition is simple in process, requires a single ingredient without compounding of other macromolecule or utilizing organic solvents like a crosslinking agent, and has wide application and popularization prospects in actual clinic application.

Description

technical field [0001] The invention relates to a method for preparing a peripheral nerve conduit by salt-induced phase transition, in particular to a preparation method for a conduit for peripheral nerve defect repair, which belongs to the field of biomedical materials. Background technique [0002] Nerve transplantation is an effective way to repair peripheral nerve damage. At present, autologous nerve transplantation is commonly used, but its clinical use is limited due to the need for surgical removal of the donor, the easy formation of neuromas in the donor site, scars, and limited nerve sources. With the development of materials science, various degradable biomaterials are used to make artificial nerve conduits to make up for the shortage of autologous nerves for nerve transplantation. The more commonly used materials include polylactic acid, polylactide, polyhydroxyalkanoate and its copolymers and other synthetic polymer materials; chitosan and its derivatives, colla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/56
CPCA61L27/20A61L27/50A61L27/56A61L2430/32C08L5/08
Inventor 魏长征杨小元宋瑞瑞蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD