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Chondrocyte composition

A chondrocyte and composition technology, applied in the field of chondrocyte composition, can solve problems such as need to be strengthened, and achieve the effects of preventing large-area migration, promoting migration, and promoting efficiency

Inactive Publication Date: 2017-02-22
SOUTH CHINA INSTITUDE OF BIOMEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current research on bioengineered tissue repairing cartilage damage still needs to be strengthened

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare the chondrocyte composition according to the following steps, and perform transplantation and repair on patients with cartilage damage:

[0030] 1. Isolation and culture of UCMSC

[0031] The fresh umbilical cords were sent to the GMP production workshop, and UCMSCs were obtained by separation. The UCMSCs were cultured and expanded; some cells were taken out to induce chondrocytes, and a certain number of chondrocytes were obtained and then expanded for transplantation.

[0032] 2. Preparation before transplantation

[0033]Digest the UCMSCs obtained above and the chondrocytes induced to differentiate with 0.25% trypsin, wash with saline, then mix the UCMSCs and chondrocytes at a ratio of 1:1 to 4:1 and resuspend them in type II collagen protein solution (the concentration of the collagen solution is 1-8% by mass), so as to obtain a cell suspension, wherein the content of collagen in the cell suspension is 1-8% by mass. The cell suspension is mixed with the t...

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PUM

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Abstract

The invention discloses chondrocyte composition. The chondrocyte composition is prepared from a cell suspension and a temperature-sensitive hydrogel, wherein the cell suspension is formed by mixing mesenchymal stem cells and chondrocyte and re-suspending the mixture in a collagen solution. The chondrocyte composition is convenient to obtain, safe and reliable, is high in chondrocyte differentiation efficiency and can provide cartilage injury parts with cells required for tissue repair; the temperature-sensitive hydrogel has good exogenous cell carrier functions, can sufficiently fill cartilage injury parts with cells in the composition and prevent the cells from moving to other parts, so that the cartilage injury parts can be effectively promoted to be rapidly and effectively repaired and healed.

Description

technical field [0001] The present invention relates to chondrocyte compositions. Background technique [0002] Articular cartilage (AC) damage is a major risk factor for osteoarthritis (OA). OA is irreversible. When the disease develops to an advanced stage, joint replacement is required to relieve pain and restore function. Since the repair capacity of articular cartilage is limited, early intervention is necessary to prevent progression to OA. Current treatment modalities aim to repair articular cartilage through primary therapy, stimulation of adjacent tissues, and grafting. Reasonable restorative treatment can achieve ideal results in the short to medium term, but after a long period of time, it is easy to relapse. In recent years, tissue engineering technology based on bioengineered tissue at the cell level has been extensively studied. This bioengineered tissue can reproduce the characteristics of hyaline cartilage and integrate with the original tissue, which is of...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/24A61L27/18A61L27/52
CPCA61L27/3834A61L27/18A61L27/24A61L27/3817A61L27/52A61L2400/06A61L2430/06C08L67/04
Inventor 裴雪涛姚海雷南雪岳文游子娟
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
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