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Preparation method of low molecular weight peptide in larva chrysomyiae

A technology of small molecular polypeptide and separation method, applied in the field of separation of traditional Chinese medicinal materials, can solve problems such as low extraction yield, achieve the effects of simple operation, good reproducibility, and avoidance of interference

Active Publication Date: 2017-02-22
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The components in the grain worm are complex and diverse, and the extraction yield is low. So far, there is no report on the separation method of the small molecule polypeptide of the traditional Chinese medicine grain worm

Method used

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  • Preparation method of low molecular weight peptide in larva chrysomyiae
  • Preparation method of low molecular weight peptide in larva chrysomyiae
  • Preparation method of low molecular weight peptide in larva chrysomyiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A. The preparation method of the small molecule polypeptide freeze-dried powder of grain worm:

[0031] 1) Homogenization: take freshly frozen grain worms, thaw and weigh them, add single distilled water 3 times the weight and volume of grain worms, and homogenize in a high-speed tissue homogenizer, and filter the homogenate with a 200-mesh gauze to obtain a filtrate;

[0032] 2) Ultrasonic crushing: Ultrasonic crushing of the filtrate for 40s, repeated 5 times, intensity 14kHz, 800W;

[0033] 3) Centrifugal filtration: Centrifuge the ultrasonically treated sample at 4°C and 12000rpm / min for 25min, take the supernatant, filter it with absorbent cotton to remove the fat in the uppermost layer, repeat this step twice to obtain the filtrate;

[0034] 4) Heating in a water bath: heat the filtrate in a water bath at 90°C while stirring for 12 minutes to remove macromolecular proteins;

[0035] 5) Take the supernatant by centrifugation: after heating the filtrate in a water ...

Embodiment 2

[0061] A. The preparation method of the small molecule polypeptide freeze-dried powder of grain worm:

[0062] 1) Homogenization: take freshly frozen grain worms, thaw and weigh them, add single-distilled water twice the weight and volume of grain worms, and homogenize in a high-speed tissue homogenizer, and filter the homogenate with a 200-mesh gauze to obtain a filtrate;

[0063] 2) Ultrasonic crushing: Ultrasonic crushing of the filtrate for 30s, repeated 4 times, intensity 14kHz, 600W;

[0064] 3) Centrifugal filtration: centrifuge the ultrasonically treated sample at 4°C and 10,000 rpm / min for 25 minutes, take the supernatant, filter it with absorbent cotton to remove the uppermost layer of fat, repeat this step twice to obtain the filtrate;

[0065] 4) Heating in a water bath: heat the filtrate in a water bath at 100°C while stirring for 12 minutes to remove macromolecular proteins;

[0066] 5) Take the supernatant by centrifugation: after heating the filtrate in a wate...

Embodiment 3

[0093] A. Preparation method of small molecule polypeptide freeze-dried powder of grain worm:

[0094] 1) Homogenization: take freshly frozen grain worms, thaw and weigh them, add single distilled water 4 times the weight and volume of grain worms, and homogenize in a high-speed tissue homogenizer, and filter the homogenate with a 200-mesh gauze to obtain a filtrate;

[0095] 2) Ultrasonic crushing: Ultrasonic crushing of the filtrate for 50s, repeated 6 times, intensity 16kHz, 800W;

[0096] 3) Centrifugal filtration: Centrifuge the ultrasonically treated sample at 4°C and 12000rpm / min for 25min, take the supernatant, filter it with absorbent cotton to remove the fat in the uppermost layer, repeat this step twice to obtain the filtrate;

[0097] 4) Heating in a water bath: heat the filtrate in a water bath at 100°C while stirring for 12 minutes to remove macromolecular proteins;

[0098]5) Take the supernatant by centrifugation: after heating the filtrate in a water bath, co...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine material separation, and particularly relates to a preparation method of low molecular weight peptide in larva chrysomyiae. The method comprises the steps of preparing freeze-dried powder of small molecules, preparing the mother solution, applying the gel chromatography for separation, conducting test to the activity of the eluent acquired from the separation, at last conducting separation to acquire the low molecular weight peptide of larva chrysomyie with anti-fungus activity. The method has the advantage of being simple, highly efficient, fast in operation, having high stability, high precision and good restoration effect. The method lays down the foundation for future research.

Description

technical field [0001] The invention belongs to the technical field of separating traditional Chinese medicinal materials, and in particular relates to a method for separating small-molecule polypeptides of grain insects. Background technique [0002] The traditional Chinese medicine maggot, also known as grain worm, narcissus, Luoxianzi, and maggot, is the larva or pupal shell of the Calliphoridae insect Chrysocephala or other closely related insects. The worm has a salty, sweet, and cold taste, and its main meridian is the spleen and stomach. Its chemical components mainly include protein, lysozyme, amino acids, alkaloids, and oils. It is recorded in "Compendium" that it can be used to treat malnutrition, malnutrition, febrile delirium, vomiting and other diseases in children. In addition, medical books "Materia Medica Qiuyuan" and "Materia Medica Biandu" also record its medicinal value. Studies have shown that some exogenous substances or physical and chemical factors ca...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/16
CPCC07K1/16C07K1/34C07K1/36
Inventor 朱家勇王敏褚夫江金小宝刘文彬
Owner GUANGDONG PHARMA UNIV
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