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Cordyceps militaris polypeptide and its preparation method and application

A technology of Cordyceps militaris and enzymolysis is applied in the field of Cordyceps militaris polypeptide and its preparation, which can solve problems such as poor drugability, and achieve the effects of low toxicity, high safety and good inhibitory effect.

Active Publication Date: 2019-09-03
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are many PTP1B inhibitors, but most of them are artificially synthesized, have great side effects, and have poor clinical druggability. So far, no PTP1B inhibitor has been clinically applied

Method used

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  • Cordyceps militaris polypeptide and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A Cordyceps militaris polypeptide is obtained by the following preparation method:

[0057] 1. Degreasing.

[0058] Take the coarse powder of the fruiting body of Cordyceps militaris, add 10 mL per gram of Cordyceps militaris, add an aqueous ethanol solution with a concentration of 95% by volume as a degreasing solution, heat and reflux in a water bath at 80 °C for 2 h, filter, and dry the filter residue at 55 °C, that is, The degreased residue of Cordyceps militaris was obtained and used for later use.

[0059] Second, enzymatic hydrolysis.

[0060] Take the above filter residues and carry out the following enzymatic hydrolysis respectively:

[0061] 1. Preparation of pepsin enzymolysis polypeptide.

[0062] The filter residue after the degreasing of above-mentioned Cordyceps militaris is added with water according to the amount of every gram of filter residue (dry weight) 10mL of water, the pH value of the solution is adjusted to 1.5, and pepsin (that is, every gra...

Embodiment 2

[0068] A Cordyceps militaris polypeptide is basically the same as the Cordyceps militaris polypeptide of Example 1, and the difference in its preparation method is:

[0069] 1. Degreasing.

[0070] Take the coarse powder of the fruiting body of Cordyceps militaris, add 10 mL per gram of Cordyceps militaris, add ethyl acetate as a degreasing solution, heat and reflux in a 60°C water bath for 2 hours, filter, and dry the filter residue at 55°C to obtain the degreasing residue of Cordyceps militaris, spare.

Embodiment 3

[0072] A Cordyceps militaris polypeptide is basically the same as the Cordyceps militaris polypeptide of Example 1, and the difference in its preparation method is:

[0073] 1. Degreasing.

[0074] Take the coarse powder of the fruiting body of Cordyceps militaris, add 10 mL per gram of Cordyceps militaris, add petroleum ether as a degreasing solution, heat to reflux in a 55°C water bath for 2h, filter, and dry the filter residue at 55°C to obtain the degreasing residue of Cordyceps militaris, which is for later use. .

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Abstract

The invention relates to cordyceps militaris polypeptide as well as a preparation method and applications of the cordyceps militaris polypeptide, belonging to the technical field of bioactive polypeptide. The preparation method of the cordyceps militaris polypeptide comprises the following steps: (1) carrying out degreasing, namely, taking cordyceps militaris, adding degreasing fluid into the cordyceps militaris, carrying out heating reflux for 1-4h at 50-80 DEG C for degreasing treatment, carrying out filtering, and carrying out drying on the filter residues for later use; and (2) carrying out enzymolysis, namely, respectively taking the filter residues, adding water into the filter residues according to the ratio that 8-25mL of water is added into each gram of filter residues, adjusting the pH value of the solution, adding with pepsase, trypsin or neutral protease, carrying out enzymolysis for 2-6 h at 30-40 DEG C, heating the enzymatic hydrolysate for inactivating the enzyme, carrying out cooling, and carrying out filtering, thus obtaining the cordyceps militaris polypeptide. The cordyceps militaris polypeptide component prepared by adopting the method has the effect of inhibiting protein tyrosine phosphatase 1B (PTP1B), and can be used for treating the diseases including insulin resistance and diabetes. Cordyceps militaris realizes the homology of medicine and food, the enzymolysis active peptide prepared by adopting the method is directly extracted from cordyceps militaris, and has the advantages of low toxicity, high safety, and long-term administration availability.

Description

technical field [0001] The invention relates to the technical field of bioactive polypeptides, in particular to a polypeptide of Cordyceps militaris and its preparation method and application. Background technique [0002] Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia. In recent years, with the continuous progress of society and the continuous improvement of people's living standards, the incidence of diabetes has also been continuously increased. Diabetes has become one of the malignant diseases that seriously endanger human life and health. Clinically, hyperglycemia is the main feature. The overall prevalence of diabetes in my country has reached 9.7%, while the prevalence of pre-diabetes is as high as 15.5%. According to the calculations made based on the survey results, the total number of people with diabetes in my country is more than 92 million, and the number of people with pre-diabetes is more than 148 million. [0003] Many ...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/56A61P5/50A61P3/10A61P3/04A23L33/18
Inventor 安丽萍杜培革苑广信候志东王曼力孙晶波赵南晰韩笑徐广宇李洪宇盛瑜郭笑杨莹莹
Owner BEIHUA UNIV
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