Multi-color super-resolution microscopy system and method based on single channel

Abstract

The invention discloses a multi-color super-resolution microscopy system and method based on a single channel. The system comprises a loss light source, a first collimating unit, a phase plate, a first excitation light source, a first dichroic mirror, a second collimating unit, a half wave plate, a polarizing beam splitter, a second excitation light source, a first detector, a second dichroic mirror, a lens-aperture-lens group, a third dichroic mirror, a scanning mirror, a second detector, a third collimating unit, a quarter wave plate, a microscopic objective and a sample stage. According to the system and method, two-color (or multi-color) super-resolution microscopy can be realized with one loss light source, and the system is simple in architecture and lower in cost. Stronger background noise caused by large energy consumption and crosstalk in a conventional single-channel multi-color super-resolution system can be overcome, the work precision is high, and the result is accurate. Therefore, compared with the prior art, the work efficiency and the experiment precision of the multi-color super-resolution microscopy system can be improved, and the cost is effectively reduced.

Description

technical field [0001] The invention relates to the fields of optical instruments and biomedical microscopic imaging, in particular to a single-channel-based multicolor super-resolution microscopic system and method. Background technique [0002] At present, research in the field of biomedicine requires higher and higher resolution. Researchers need to understand the three-dimensional structure of various microscopic substances. However, the spot size of traditional white-light wide-field microscopes and laser confocal microscopes cannot achieve such resolution. , and the emergence of super-resolution microscopy system perfectly solves this problem. In super-resolution microscopy, the current research hotspots mostly focus on various fluorescence microscopy techniques, such as stimulated emission depletion (STED) fluorescence microscopy and single-molecule localization fluorescence microscopy (SMS). Among them, there are two types of light in the STED system: excitation lig...

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a novel multi-color super-resolution microscopic system based on a single channel, aiming at problems such as crosstalk or complex structure existing in the previous multi-color super-resolution microscopic system

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