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A method for the analysis of linoleic acid metabolites in the process of maltosaccharification

An analysis method and technology of saccharification process, applied in the direction of analysis of materials, material separation, instruments, etc., can solve the problems of inability to fully and effectively study the formation mechanism of trans-2-nonenal, the unstable existence of precursors, etc., and achieve improvement Stability and drinkability, improvement of antioxidant capacity, effect of controlling beer aging

Active Publication Date: 2018-11-27
TSINGTAO BREWERY
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Problems solved by technology

Due to the unstable existence of its precursors, it is not possible to fully and effectively study the formation mechanism of trans-2-nonenal

Method used

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  • A method for the analysis of linoleic acid metabolites in the process of maltosaccharification
  • A method for the analysis of linoleic acid metabolites in the process of maltosaccharification
  • A method for the analysis of linoleic acid metabolites in the process of maltosaccharification

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Embodiment 1

[0048] 50 μL of 99% pure 13 Add C-labeled linoleic acid to 10g of malt powder, add 20mL of distilled water, and saccharify it according to the agreed wort method at 47°C; Minutes, 50ml of fermentation broth in the final 15-30 minutes, centrifuged at 12000rpm for 8 minutes, vacuum freeze-dried the supernatants obtained after centrifugation respectively, and obtained intermediate powders 1, 2, 3 in turn; to intermediate powders 1, 2, 3 100 μL of 25 mg / mL hydroxylamine pyridine hydrochloride solution was added to the solution, and after heating at 65°C for 0.75 hours, 60 μL of derivatization reagents were added respectively, and then heated at 65°C for 1.5 hours, taken out and cooled to room temperature naturally, and the labeled dry powder 1, 2, 3; replaced by standard linoleic acid 13 C-marked linoleic acid, repeat the above steps to obtain standard dry powders 1', 2', 3' corresponding to marked dry powders 1, 2, 3; 2, 3 and standard dry powder 1', 2', 3' were qualitatively d...

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Abstract

The invention provides a method for analyzing linoleic acid metabolites in the malt saccharifying process. The generation mechanism of trans-2-nonenal is comprehensively and effectively researched, beer aging can be controlled, and the potability of beer is improved. The analyzing method includes the steps that marked dry powder obtained through linoleic acid marked with 13C and standard dry powder obtained through unmarked linoleic acid are respectively and qualitatively detected through a gas chromatograph-mass spectrometer, the result is compared with a database, analysis is carried out, and the different linoleic acid metabolites are obtained. According to the method, precursors for generating the trans-2-nonenal can be obtained through analysis, the generating mechanism of the trans-2-nonenal can be more objectively and comprehensively recognized, and therefore a main processing measure for pertinently improving the oxidation resistance of beer can be achieved.

Description

technical field [0001] The invention relates to an analysis method, in particular to an analysis method of linoleic acid metabolites in the maltosaccharification process, especially an analysis method of trans-2-nonenal precursor in the linoleic acid metabolites. Background technique [0002] Beer is one of the most ubiquitous products in the beverage market, and the stability of its taste and flavor is an important quality factor controlled by the manufacturer. However, beer ages from the moment fermentation ends. Generally speaking, aging originates from the end of beer fermentation and changes with the properties of raw materials and storage conditions. Carbonyl compounds are often indicators of aging, especially aldehydes with low odor thresholds. These aldehydes are derived from the oxidation of amino acids, alcohols and fatty acids. With an odor threshold of only 0.1 ppb, trans-2-nonenal is widely recognized as an important indicator of aging. [0003] At present, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 董建军袁宪正余俊红韩振莲尹花段建鲁常宗明郭荣波胡淑敏黄淑霞杨梅
Owner TSINGTAO BREWERY
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