The invention discloses gynostemma pentaphylla sheep-bone oil seasoning powder. The seasoning powder is prepared from, by weight, 4-5 parts of high protein oxidized sheep-bone oil, 100-105 parts of sheep-bone essence enzymatic hydrolysate, 0.9-1 part of cysteine, 0.9-1 part of glycine, 1.8-2 parts of alanine, 0.9-1 part of proline, 2.4-2.5 parts of xylose, 1.2-1.3 parts of glucose, 11.8-12 parts of VB, 5-7 parts of wheat berries, 8-9 parts of fish eggs, 4-5 parts of Chinese dates, 10-13 parts of cactus, 2-2.3 parts of gynostemma pentaphylla, 1.2-2.5 parts of apple leaves and 2-3 parts of bunge cherry seeds. According to the gynostemma pentaphylla sheep-bone oil seasoning powder and the preparation method thereof, the oxidized sheep-bone oil is added, the variety of volatile flavoring substances of a mutton flavor seasoning base material can be remarkably influenced, flavor of the thermal reaction type mutton flavor seasoning base material is enhanced, for example, trans-2, 4-decadienal and trans-2-nonenal are the key volatile flavoring substances for improving the seasoning base material, and sweet fragrance, delicate fragrance, scorch aroma, citrus fragrance and other fragrance are contained.