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Electrochemical luminescence mark liquid

A luminescent labeling and electrochemical technology, which is applied in the directions of labels, scientific instruments, measuring devices used in chemical analysis, etc., can solve the problem of low luminous efficiency of electrochemiluminescence labeling liquid, improve the unit luminescence amount, reduce the cost of labeling, The effect of improving the luminous efficiency

Active Publication Date: 2017-02-22
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention solves the problem of low luminous efficiency of the existing electrochemiluminescent marking liquid, and aims to provide an electrochemiluminescent marking liquid with high luminous efficiency, good stability and rapid response

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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with specific embodiments.

[0018] An electrochemiluminescent labeling solution, which is formed by mixing ruthenium terpyridine and a buffer substance, and is prepared from the following raw materials according to the following weight-to-volume ratio:

[0019]

[0020] Wherein, the terpyridine ruthenium is 2,2':6',2"-Terpyridine[4'-(N-succinyloxycarbonylpropyl)-2,2':6',2"-terpyridine]( 2,2':6',2"-terpyridine) ruthenium, the structural formula is:

[0021]

[0022] One, embodiment formula:

[0023]

[0024] 2. Application fields and methods:

[0025] The invention is applied to the development of the electrochemiluminescence immunoassay kit, and is directly applied to the antibody labeling process after preparing the labeling solution according to the formula.

[0026] 3. How to use:

[0027] The method of use is as follows: at room temperature, take 1ml antibody (1mg / ml) and ad...

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Abstract

The invention provides electrochemical luminescence mark liquid. The electrochemical luminescence mark liquid is prepared by mixing tris bipyridine ruthenium, buffer substances and distilled water. The electrochemical luminescence mark liquid is mainly prepared from, by weight, 0.22-0.52 g of tris bipyridine ruthenium, 1.88-3.02 g of disodium hydrogen phosphate and 2.06-3.18 g of monopotassium phosphate, the distilled water is added till the volume is kept at 300 ml, and the PH value is adjusted to range from 6.5 to 7.6. The electrochemical luminescence mark liquid is composed of a single solution, 2,2':6',2''-Terpyridine[4'(N-succinyl oxo carbonyl propyl)-2,2':6',2''-terpyridyl](2,2':6',2''-terpyridyl)ruthenium(II) is adopted as a marker, and the unit luminous quantity is greatly increased; by means of the high conjugation effect of molecules, higher detection sensitivity is achieved; trisodium citrate is added, the solubility of labeled protein is improved, the marking rate is increased, the marking cost is reduced accordingly, and meanwhile the luminous quantity of unit concentration reactants is increased.

Description

technical field [0001] The invention belongs to the field of reagents for medical diagnostic instruments, and in particular relates to an electrochemiluminescence marking liquid. Background technique [0002] At present, the commonly used methods for the quantitative detection of disease-related trace substances in the human body include chemiluminescence immunoassay and electrochemiluminescence immunoassay. The precision is high, and the reagent is stable, and it can be analyzed fully automatically, so it is an ideal marker immunoassay method. Electrochemiluminescence immunoassay has been widely used for its high sensitivity and good repeatability. Electrochemiluminescence immunoassays are divided into luminescent systems of organic compounds and luminescent systems of inorganic substances. Organic luminescent substances include hydrazide compounds, acridinium esters and lucigenin, etc. In the electrochemiluminescent system of inorganic compounds, the most typical lumines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531G01N2458/30
Inventor 廖璞潘能科
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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