An electrochemiluminescence labeling solution

A luminescent labeling and electrochemical technology, applied in the directions of labels, scientific instruments, and measuring devices used in chemical analysis, can solve the problems of low luminous efficiency and poor stability of electrochemiluminescence labeling liquid, and achieve high detection sensitivity , The effect of increasing the amount of light per unit and reducing the cost of marking
CN106442965BActive Publication Date: 2018-02-23CHONGQING ZHONGYUAN BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING ZHONGYUAN BIOLOGICAL TECH
Publication Date
2018-02-23

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Abstract

The invention provides electrochemical luminescence mark liquid. The electrochemical luminescence mark liquid is prepared by mixing tris bipyridine ruthenium, buffer substances and distilled water. The electrochemical luminescence mark liquid is mainly prepared from, by weight, 0.22-0.52 g of tris bipyridine ruthenium, 1.88-3.02 g of disodium hydrogen phosphate and 2.06-3.18 g of monopotassium phosphate, the distilled water is added till the volume is kept at 300 ml, and the PH value is adjusted to range from 6.5 to 7.6. The electrochemical luminescence mark liquid is composed of a single solution, 2,2':6',2''-Terpyridine[4'(N-succinyl oxo carbonyl propyl)-2,2':6',2''-terpyridyl](2,2':6',2''-terpyridyl)ruthenium(II) is adopted as a marker, and the unit luminous quantity is greatly increased; by means of the high conjugation effect of molecules, higher detection sensitivity is achieved; trisodium citrate is added, the solubility of labeled protein is improved, the marking rate is increased, the marking cost is reduced accordingly, and meanwhile the luminous quantity of unit concentration reactants is increased.
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Description

technical field

[0001] The invention belongs to the field of reagents for medical diagnostic instruments, and in particular relates to an electrochemiluminescence marking liquid. Background technique

[0002] At present, the commonly used methods for the quantitative detection of disease-related trace substances in the human body include chemiluminescence immunoassay and electrochemiluminescence immunoassay. The precision is high, and the reagent is stable, and it can be analyzed fully automatically, so it is an ideal marker immunoassay method.

[0003] However, electrochemiluminescence immunoassay has been widely used for its high sensitivity (up to 10-11~10-17mol / L) and good repeatability. Electrochemiluminescence immunoassays are divided into luminescent systems of organic compounds and luminescent systems of inorganic substances. Organic luminescent substances include hydrazide compounds, acridinium esters and lucigenin, etc. In the electrochemiluminescent system of in...

Claims

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