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An electrochemiluminescence labeling solution

A luminescent labeling and electrochemical technology, applied in the directions of labels, scientific instruments, and measuring devices used in chemical analysis, can solve the problems of low luminous efficiency and poor stability of electrochemiluminescence labeling liquid, and achieve high detection sensitivity , The effect of increasing the amount of light per unit and reducing the cost of marking

Active Publication Date: 2018-02-23
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention solves the problems of poor stability and low luminous efficiency of the existing electrochemiluminescent marking liquid, and aims to provide an electrochemiluminescent marker with good stability, rapid response and high luminous efficiency. Marking fluid

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  • An electrochemiluminescence labeling solution
  • An electrochemiluminescence labeling solution
  • An electrochemiluminescence labeling solution

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Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with specific embodiments.

[0019] An electrochemiluminescent labeling solution, comprising ruthenium terpyridine, buffer substances and distilled water, prepared from the following raw materials in the following weight-to-volume ratio:

[0020]

[0021] Wherein, the structural formula of the terpyridine ruthenium is:

[0022]

[0023] One, embodiment formula:

[0024]

[0025]

[0026] 2. Application fields and methods:

[0027] The invention is applied to the development of the electrochemiluminescence immunoassay kit, and is directly applied to the antibody labeling process after preparing the labeling solution according to the formula.

[0028] 3. How to use:

[0029] The method of use is as follows: at room temperature, take 1ml antibody (1mg / ml) and add 10uL of the above labeling solution, mix slightly and react in a 37°C water bath for 30 minutes. After taking it out, c...

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Abstract

The invention provides electrochemical luminescence mark liquid. The electrochemical luminescence mark liquid is prepared by mixing tris bipyridine ruthenium, buffer substances and distilled water. The electrochemical luminescence mark liquid is mainly prepared from, by weight, 0.22-0.52 g of tris bipyridine ruthenium, 1.88-3.02 g of disodium hydrogen phosphate and 2.06-3.18 g of monopotassium phosphate, the distilled water is added till the volume is kept at 300 ml, and the PH value is adjusted to range from 6.5 to 7.6. The electrochemical luminescence mark liquid is composed of a single solution, 2,2':6',2''-Terpyridine[4'(N-succinyl oxo carbonyl propyl)-2,2':6',2''-terpyridyl](2,2':6',2''-terpyridyl)ruthenium(II) is adopted as a marker, and the unit luminous quantity is greatly increased; by means of the high conjugation effect of molecules, higher detection sensitivity is achieved; trisodium citrate is added, the solubility of labeled protein is improved, the marking rate is increased, the marking cost is reduced accordingly, and meanwhile the luminous quantity of unit concentration reactants is increased.

Description

technical field [0001] The invention belongs to the field of reagents for medical diagnostic instruments, and in particular relates to an electrochemiluminescence marking liquid. Background technique [0002] At present, the commonly used methods for the quantitative detection of disease-related trace substances in the human body include chemiluminescence immunoassay and electrochemiluminescence immunoassay. The precision is high, and the reagent is stable, and it can be analyzed fully automatically, so it is an ideal marker immunoassay method. [0003] However, electrochemiluminescence immunoassay has been widely used for its high sensitivity (up to 10-11~10-17mol / L) and good repeatability. Electrochemiluminescence immunoassays are divided into luminescent systems of organic compounds and luminescent systems of inorganic substances. Organic luminescent substances include hydrazide compounds, acridinium esters and lucigenin, etc. In the electrochemiluminescent system of in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531
CPCG01N33/531G01N2458/30
Inventor 廖璞潘能科
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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