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Application of five plant growth-promoting rhizobacteria to improvement of agronomic traits of plants

A technology for rhizosphere growth-promoting bacteria and agronomic traits, applied in the field of biotechnology and breeding, can solve the problems of difficult to be recognized by consumers, not obvious effect, difficult to popularize and apply, etc.

Active Publication Date: 2017-03-08
SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the rhizosphere growth-promoting bacteria have no obvious effect on promoting plant growth and development, and are difficult to be recognized by consumers; some rhizosphere growth-promoting bacteria only have a growth-promoting effect on one or several plants, so Difficult to be popularized

Method used

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  • Application of five plant growth-promoting rhizobacteria to improvement of agronomic traits of plants
  • Application of five plant growth-promoting rhizobacteria to improvement of agronomic traits of plants
  • Application of five plant growth-promoting rhizobacteria to improvement of agronomic traits of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Example 1 Determination of beneficial bacteria for promoting plant growth and development

[0123] Soil samples were collected from the millet test field in Yangling Agricultural High-tech Industrial Demonstration Park (34°16′18″N / 108°4′59″E), Shaanxi Province. When the millet was harvested, 3 millets of each line were randomly selected, and Bulgarelli et al. (2012) (Bulgarelli, D., Rott, M., Schlaeppi, K., van Themaat, E.V.L., Ahmadinejad, N., Assenza, F. ,...&Schulze-Lefert, P. (2012). Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota. Nature, 488(7409), 91-95.) The method described in collecting rhizosphere soils. Meanwhile, take 10 parts of soil in an unplanted plot. Put the collected soil samples into sterilized collection tubes, freeze them on dry ice and transport them to Shenzhen Huada Gene Research Institute, and store them in a -20°C low-temperature refrigerator for later use.

[0124] Take 0.2 g of each sample for ge...

Embodiment 2

[0130] Embodiment 2 selects bacterial strain, prepares agricultural preparation

[0131] 2.1 Source of strain

[0132] According to the genus information obtained in Example 1 of the present invention, the inventor BLAST compares the 16S rRNA gene sequences of multiple strains of yellow soil-derived bacteria with OUT255, and the comparison results are shown in Table 1; the 16S rRNA gene sequences of multiple strains of Ferruginibacter bacteria Perform BLAST comparison with OTU5352, and the comparison results are shown in Table 2; BLAST comparison of the 16S rRNA gene sequences of multiple strains of Rendzella with OTU1892, and the comparison results are shown in Table 3; The rRNA gene sequence was compared with OTU414 by BLAST, and the comparison results were shown in Table 4;

[0133] Table 1 BLAST comparison results of OTU255 sequence and 16S rRNA gene partial sequences of four yellow soil-derived bacteria

[0134]

[0135]

[0136] Table 2 BLAST comparison results o...

Embodiment 3

[0175] Example 3 Effects of five rhizosphere growth-promoting bacteria on millet germination under laboratory conditions

[0176] experimental method:

[0177] 3.1 Seed Treatment

[0178] Millet seeds were surface sterilized with 1% sodium hypochlorite for 5 minutes and rinsed 5 times with sterile distilled water. Using 1% carboxymethyl cellulose as an adhesive to coat the seed coat, the ingredients of the seed coat are Flavisolibacter, Ferruginibacter, Lentzea, Solirubrobacter, Terrimonas, or a combination thereof, and a mixture of peat (Biocare Technology Pvt., Australia) (bacteria content of 10 8 -10 9 CFU / g peat) and then air-dried. 10 bacteria per seed 6 -10 7 CFU. Control seeds were wrapped with non-bacterial peat.

[0179] 3.2 In vitro growth promotion test

[0180] Bacterial-treated and control seeds were sown onto paper towels. Soak paper towels in distilled water and place 50 seeds evenly on each paper towel. Then cover it with another damp paper towel to ...

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Abstract

The invention provides application of five plant growth-promoting rhizobacteria to improvement of the agronomic traits of plants. Specifically, the invention discloses application of the plant growth-promoting rhizobacteria to preparation of an agricultural preparation. The agricultural preparation is used for improving the agronomic traits of plants. The plant growth-promoting rhizobacteria are selected from a group of consisting of Flavisolibacter, Ferruginibacter, Lentzea, Solirubrobacter and Terrimonas or a composition thereof. The five plant growth-promoting rhizobacteria and the composition thereof can (i) promote seed germination, (ii) increasing plant height, (iii) promoting root growth, (iv) increasing a leaf area, (v) improving a tiller number and (vi) improving output; so the five plant growth-promoting rhizobacteria and the composition have good application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology and breeding, in particular, the invention relates to rhizosphere growth-promoting bacteria (Flavisolibacter, Ferruginibacter, Lentzea, Solirubrobacter), The use of Terrimonas in improving the agronomic traits of plants. Background technique [0002] Fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate decreases year by year. The residues of pesticides and fertilizers have released a large amount of harmful substances into the environment, polluting soil, water sources and food. It poses a great threat to human separation and living environment, and calls for protecting the ecological environment and producing safe food are growing stronger. Therefore, the development and utilization of new fertilizer sources to replace chemical fertilizers in order to adapt to the development of green agriculture and gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P21/00C05F11/08C05G3/00
Inventor 金桃冯强万景旺王俊
Owner SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH
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