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Rice seed fast germination QTL qGS11 molecular marker and application thereof

A technology of molecular markers and rice seeds, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effects of high selection efficiency, fast and simple identification methods, and low reliability

Active Publication Date: 2017-03-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] By constructing different rice genetic populations, many scholars have located multiple QTLs related to the control of seed germination, but only one main QTL qLTG3-1 controlling low-temperature germination of rice seeds has been successfully cloned

Method used

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  • Rice seed fast germination QTL qGS11 molecular marker and application thereof
  • Rice seed fast germination QTL qGS11 molecular marker and application thereof
  • Rice seed fast germination QTL qGS11 molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Materials and methods:

[0031] 1. Materials: Parental rice variety IR28 with high vigor and rice variety with low vigor Daguan rice, and BC constructed with IR28 as the recurrent parent 1 f 2 and BC 5 f 2 group.

[0032] 2. Extract individual DNA by SDS method.

[0033] 3. Molecular marker development: New primers were designed with Nipponbare and 9311 as the reference sequence, and the parental IR28 and Ozeki rice were used as templates for PCR amplification to screen for polymorphic markers.

[0034] 4. PCR reaction system: the volume is 25 microliters, including 2.5 microliters of 10×buffer, 25mM MgCl 2 1.5 microliters, 2.5 microliters of 4 pmol / microliter primer pair, 2 microliters of 2.5mM dNTPs, 0.2 microliters of 5 units / microliter Taq enzyme, 20 nanograms of template DNA, and add water to 25 microliters. The reaction program was DNA pre-denaturation at 95°C for 5 min; pre-denaturation at 95°C for 30 s, annealing at 50°C for 30 s, extension at 72°C fo...

Embodiment 2

[0043] (1) Materials and methods:

[0044] 1. Materials: rice variety IR28 and Daguan rice

[0045] 2. Extract individual DNA by SDS method.

[0046] 3. Marks: YB20, YB21 and RM26614.

[0047] 4. PCR reaction system: the volume is 25 microliters, including 2.5 microliters of 10×buffer, 25mM MgCl 2 1.5 microliters, 2.5 microliters of 4 pmol / microliter primer pair, 2 microliters of 2.5mM dNTPs, 0.2 microliters of 5 units / microliter Taq enzyme, 20 nanograms of template DNA, and add water to 25 microliters. The reaction program was DNA pre-denaturation at 95°C for 5 min; pre-denaturation at 95°C for 30 s, annealing at 50°C for 30 s, extension at 72°C for 30 s, and 35 cycles; finally, extension at 72°C for 10 min. PCR amplification was carried out on a biometer amplification instrument, and the amplified products were separated by electrophoresis on 8% non-denaturing polyacrylamide gel (containing 7.6 g of acrylamide and 0.4 g of methylenebisacrylamide in 100 ml of polyacrylami...

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Abstract

The invention discloses a rice seed fast germination QTL qGS11 molecular marker and application thereof. One or more primer pairs marked by SSR (simple sequence repeat) are adopted for PCR (polymerase chain reaction) amplification of rice genomic DNA, a PCR amplification product is subjected to electrophoresis detection on 8% non-denaturing polyacrylamide gel, and if a DNA fragment in corresponding size is obtained by amplification, existence of rice seed fast germination QTL qGS11 enhancing allele is determined. By a molecular marker method in coseparation and close linkage with a seed fast germination gene qGS11, rice seed germination speed can be predicted, and high-activity rice varieties can be screened out quickly.

Description

technical field [0001] The invention belongs to the field of seed science and technology application, and relates to a molecular marker of rice seed rapid germination QTL qGS11 and its application. Background technique [0002] In agricultural production, abiotic stresses such as low temperature and floods are often encountered after sowing. Low vigor seeds germinate slowly, have a low emergence rate and poor resistance, resulting in field seedling rot, necrosis and overgrown weeds, which eventually lead to severe production reduction. At the same time, with the increasing shortage of labor force, the promotion of direct-directed rice is becoming more and more widespread. Therefore, it is of great significance to increase the germination speed of seeds and to breed rice varieties with high vigor. It is a very effective method to select genes / QTLs related to rapid germination of rice seeds by excavating and positionally cloning them and using molecular marker-assisted select...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王州飞张红生程金平杨彬王建飞黄骥鲍永美
Owner NANJING AGRICULTURAL UNIVERSITY