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AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine

A technology for ractopamine and detection kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of high instrument cost, low detection sensitivity, and cumbersome operation

Inactive Publication Date: 2017-03-08
INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have disadvantages such as cumbersome operation, low detection sensitivity, and high instrument cost.

Method used

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  • AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine
  • AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine
  • AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine

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Embodiment Construction

[0016] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0017] The anti-clenbuterol monoclonal antibody and anti-ractopamine monoclonal antibody in the kit can be purchased from the market, or can be prepared by conventional methods for preparing monoclonal antibodies.

[0018] 1 Preparation of biotinylated antigen

[0019] The preparation process of biotinylated antigen was as follows: 32 μL each of clenbuterol antigen and ractopamine antigen and 2 μL biotin were added to a 250 μL centrifuge tube, then mixed by vortex shaker, and incubated in the dark for 90 min, after storage in a 4 °C refrigerator. A biotinylated antigen is obtained.

[0020] 2 Exploration of reaction conditions

[0021]...

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Abstract

The invention discloses an AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine. The AlphaLISA detection kit comprises a clenbuterol standard product, a ractopamine standard product, biotinylated clenbuterol, biotinylated ractopamine, an anti-clenbuterol monoclonal antibody, an anti-ractopamine monoclonal antibody, streptavidin donor micro beads, anti-mouse IgG coated receptor micro beads, a detection buffer solution, a white reaction plate and a sealing film, wherein the streptavidin donor micro beads comprise 100mu g / ml donor micro beads, and the anti-mouse IgG coated receptor micro beads comprise 100 mu g / ml receptor micro beads. By use of the kit disclosed by the invention, an AlphaLISA detection method for detecting clenbuterol and ractopamine in animal-origin muscle tissues is established, and the detection method has the advantages of simplicity, rapidness, low sample dosage, high sensitivity, no need of washing, and the like.

Description

technical field [0001] The invention relates to the detection of clenbuterol and ractopamine in animal-derived muscle tissue, in particular to an AlphaLISA detection kit for simultaneously detecting clenbuterol and ractopamine in animal-derived muscle tissue. Background technique [0002] Clenbuterol and ractopamine are beta-agonists with similar chemical structures and functions. Clenbuterol and ractopamine can be used in the treatment of diseases and as growth promoters and fattening agents to reduce fat content and increase muscle protein synthesis. However, they can have serious effects on the human body, such as heart palpitations, headaches, etc. Existing methods for detecting clenbuterol and ractopamine include immunosorbent assay, electrochemical detection method, fluorescent probe method, high performance liquid chromatography (HPLC), ultratrace LC-MS / MS, gas chromatography-mass spectrometry (GC-MS), etc. These methods are very reliable and important. However, t...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/533
CPCG01N33/577G01N33/533G01N33/54313
Inventor 郗存显聂福平曹淑瑞张雷王国民李贤良唐柏彬郑小玲王雄
Owner INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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