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Inhibitors of sterol metabolism for their use to accumulate triglycerides in microalgae, and methods thereof

An inhibitor, microalgae technology, applied in the production of fatty acids, pharmaceutical or cosmetic compositions, biofuels, food supplements, and can solve problems such as low yield, growth restriction, lack, etc.

Active Publication Date: 2017-03-08
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phaeodactylum tricornutum is currently used industrially for the production of omega-3 polyunsaturated fatty acids, but this application, as well as others such as biofuels, is limited when TAG accumulation is induced by conventional nutrient deficient methods such as nitrogen starvation. Industrial implementation is still limited by growth retardation and low yields of biomass (Chisti, Journal of Biotechnology 167(2013) 201-214)
These methods have an important drawback: nitrogen deficiency leads to growth limitation

Method used

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  • Inhibitors of sterol metabolism for their use to accumulate triglycerides in microalgae, and methods thereof
  • Inhibitors of sterol metabolism for their use to accumulate triglycerides in microalgae, and methods thereof
  • Inhibitors of sterol metabolism for their use to accumulate triglycerides in microalgae, and methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0103] 1) Materials and methods

[0104] 1) 1-Phaeodactylum tricornutum strain and growth conditions

[0105] Phaeodactylum tricornutum (Ptl) Bohlin strain 8.6CCMP2561 (Collection of Marine Organisms, now known as NCMA: National Center for Marine Algae and Microbiota) was used in each experiment.

[0106] Pt1 was grown in "enriched artificial seawater" (ESAW) medium prepared according to the specifications of the Canadian Center for the Culture of Microorganisms in a 250 mL flask at 20°C.

[0107] To prepare the ESAW medium, four separate solutions were prepared: two salt solutions (Solution 1 and Solution 2), one nutrient solution and one vitamin solution. The salt was added to distilled deionized water (DDW). When the salts in Solution 1 and Solution 2 are completely dissolved, mix Solution 1 and Solution 2 together. Dilute the total volume with DDW.

[0108] table 3 : Composition of ESAW Salt Solution 1 and ESAW Salt Solution 2

[0109]

[0110] Table 4 : Nutr...

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Abstract

The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.

Description

technical field [0001] The invention relates to a method for accumulating triacylglycerol in microalgae by inhibiting sterol metabolism. The invention also relates to a method for the production of fatty acids, biofuels, pharmaceutical or cosmetic compositions, and food supplements, said method comprising the step of accumulating triacylglycerols in microalgae according to the invention. Finally, the present invention concerns the use of inhibitors of sterol metabolism for the accumulation of triglycerides in microorganisms, and preferably microalgae. Background technique [0002] It is known that crops used for nutritional purposes cannot be converted to crops used for oilseed production (Durrett et al., The Plant Journal (2008) 54, 593-607). Therefore, efforts have been made to perform oil production in other organisms such as algae (Chisti, Biotechnology Advances 25 (2007) 294-306; Chisti, Trends in Biotechnology, 2008, Vol 26, No. 3; Dismukes et al., Current Opinion in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P7/6458C12P7/649
CPCC12P7/649C12P7/6463C12P7/6458Y02E50/10A23L33/12A23V2002/00C10L1/02C10L2200/0469C10L2290/26C10L2290/544
Inventor 美利莎·康特利娜-朱厄纳·多尔希科琳·梅卡罗琳·巴雷特迪米特里斯·佩特劳特索斯丹尼斯·法尔科内特朱丽叶·茹赫特法布里斯·瑞贝耶让-克里斯托夫·桑特拉埃里克·马雷查尔
Owner COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES