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Low-serum high-efficiency culture medium for culturing Mycoplasma hyopneumoniae and preparation method thereof

A technology of mycoplasma hyopneumoniae and low serum, which is applied in the biological field, can solve the problems of easy secondary virus or bacterial infection, lower feed conversion rate, poor growth and development, etc., and achieve the goal of increasing antigen titer, quality improvement, and stable growth titer Effect

Active Publication Date: 2020-02-07
内蒙古金源康生物工程股份有限公司
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  • Abstract
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Problems solved by technology

In addition to reducing the feed conversion rate and poor growth and development, pigs are prone to secondary virus or bacterial infection after infection, resulting in increased mortality of pigs and heavy economic losses.

Method used

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  • Low-serum high-efficiency culture medium for culturing Mycoplasma hyopneumoniae and preparation method thereof
  • Low-serum high-efficiency culture medium for culturing Mycoplasma hyopneumoniae and preparation method thereof
  • Low-serum high-efficiency culture medium for culturing Mycoplasma hyopneumoniae and preparation method thereof

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] The low-serum high-efficiency culture medium 1 for culturing Mycoplasma hyopneumoniae includes a basic medium and an additional package. The basic medium includes: M199, brain extract powder, hydrolyzed milk protein, BSA, glucose, fresh yeast extract, Hepes, ferric citrate, calcium chloride. 2H 2 O, phenol red, ddH 2 O. The components of the additive package include cholesterol, linoleic acid, linolenic acid, palmitic acid, ethanol and pig serum.

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Abstract

The invention provides a low-serum efficient culture medium for culturing mycoplasma hyopneumoniae and a preparation method thereof. The low-serum efficient culture medium comprises a base medium and an addition bag. The base medium comprises M199, brain extract powder, lactoalbumin hydrolysate, BSA, glucose, fresh yeast extract, Hepes, iron citrate, calcium chloride, 2H2O, phenol red and dd H2O. The addition bag comprises cholesterol, linoleic acid, linolenic acid, palmitic acid, ethanol and porcine serum. Through use of some hydrolyzates and lipid, a serum addition ratio is less than 5% so that a cost is reduced, the quality of mycoplasma is improved, and mycoplasma hyopneumoniae antigen titer is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a culture medium for cultivating Mycoplasma hyopneumoniae with low serum and high efficiency. Background technique [0002] Mycoplasma hyopneumoniae can cause chronic respiratory diseases in pigs and cause economic losses in the pig industry. The cultivation and storage conditions of Mycoplasma pneumoniae are very harsh, which is a technical problem for large-scale vaccine production. Mycoplasma hyopneumoniae, also known as swine asthma, is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae (Mhp), which is widely distributed all over the world. Infection of pigs not only leads to lower feed conversion rate, poor growth and development, but also is prone to secondary virus or bacterial infection, resulting in increased mortality of pigs and heavy economic losses. Vaccination is the most important means of preventing this disease. Among the component...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/35
CPCC12N1/20
Inventor 赵峻岭
Owner 内蒙古金源康生物工程股份有限公司
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