Medium and method for colorful-leaf euonymus japonicus tissue culture

A tissue culture and culture medium technology, which is applied to the medium field of golden armor tissue culture, can solve the problems of unsuitability for golden armor culture, low reproduction coefficient and survival rate, etc., so as to shorten the reproduction cycle, improve the reproduction coefficient, and maintain excellent characters. Effect

Active Publication Date: 2017-03-22
河南红枫生物高科股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But above-mentioned two kinds of tissue culture methods are not suitable for the cultivation of golden nails, adopt above-men

Method used

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  • Medium and method for colorful-leaf euonymus japonicus tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Tissue culture of golden nail

[0047] 1. Growth environment

[0048] (1) Using solid WPM medium, the prepared medium is sterilized by high-pressure and high-temperature steam at 126°C for 5 minutes.

[0049] (2) In the tissue culture room, the light time is 12h / d, the light intensity is 3500lx, the culture temperature is 25℃, the relative humidity is 48%, and it is sterilized by ultraviolet light every 3 days for 30 minutes.

[0050] 2. Treatment of explants

[0051] (1) From May to June every year, young shoots with healthy growth and disease-free pests are taken as explants.

[0052] (2) After the collection is completed, first remove the leaves to retain the petioles, and then wrap them with gauze and rinse them under running water for 2 hours.

[0053] (3) Dip a soft brush with a solution containing 0.1% NaClO to clean the explants.

[0054] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of uses...

Example Embodiment

[0083] Example 2 Tissue culture of golden nails

[0084] 1. Growth environment

[0085] (1) Using solid WPM medium, the prepared medium is sterilized by high-pressure and high-temperature steam at 126°C for 5 minutes.

[0086] (2) In the tissue culture room, the light time is 12h / d, the light intensity is 3500lx, the culture temperature is 25℃, the relative humidity is 48%, and the ultraviolet light is sterilized every 3 days for 30 minutes.

[0087] 2. Treatment of explants

[0088] (1) From May to June every year, young shoots with healthy growth and disease-free pests are taken as explants.

[0089] (2) After the collection is completed, first remove the leaves to retain the petioles, and then wrap them with gauze and rinse them under running water for 2 hours.

[0090] (3) Dip a soft brush with a solution containing 0.1% NaClO to clean the explants.

[0091] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of uses ...

Example Embodiment

[0120] Example 3 Tissue culture of golden nails

[0121] 1. Growth environment

[0122] (1) Using solid WPM medium, the prepared medium is sterilized by high-pressure and high-temperature steam at 126°C for 5 minutes.

[0123] (2) In the tissue culture room, the light time is 12h / d, the light intensity is 3500lx, the culture temperature is 25℃, the relative humidity is 48%, and the ultraviolet light is sterilized every 3 days for 30 minutes.

[0124] 2. Treatment of explants

[0125] (1) From May to June every year, young shoots with healthy growth and disease-free pests are taken as explants.

[0126] (2) After the collection is completed, first remove the leaves to retain the petioles, and then wrap them with gauze and rinse them under running water for 2 hours.

[0127] (3) Dip a soft brush with a solution containing 0.1% NaClO to clean the explants.

[0128] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of uses ...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a medium and method for colorful-leaf euonymus japonicus tissue culture. The medium comprises a primary medium, a first subculture medium, a second subculture medium and a rooting medium. By means of the formula of the medium, the propagation coefficient of colorful-leaf euonymus japonicus can be remarkably improved, the propagation period is shortened, the survival rate is increased, and a more effective means is provided for industrial production of colorful-leaf euonymus japonicus.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of golden nails. Background technique [0002] Golden armor is a variant species of Euonymus japonicus cv. "Cu Zhi" in the Euonymus family Euonymus. Its leaves are golden on both sides and emerald green in the middle, hence the name. The golden beetle has a compact plant type and bright color, and inherits the characteristics of good cold resistance and resistance of the Hokkaido boxwood. It is a colorful non-deciduous plant that is lacking in the north. Golden beetle has strong uprightness, strong sprouting ability, high transplanting survival rate, and is easy to maintain. It can be cultivated into various shapes such as hedges, balls, and green walls. It is a garden tree species with great market potential. At present, the golden beetle is mainly propagated by grafting, which is time-consuming and laborious, and has a low reprod...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂何甜甜郭冠男孟伟芳
Owner 河南红枫生物高科股份有限公司
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