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Medium and method for colorful-leaf euonymus japonicus tissue culture

A tissue culture and culture medium technology, which is applied to the medium field of golden armor tissue culture, can solve the problems of unsuitability for golden armor culture, low reproduction coefficient and survival rate, etc., so as to shorten the reproduction cycle, improve the reproduction coefficient, and maintain excellent characters. Effect

Active Publication Date: 2017-03-22
河南红枫生物高科股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But above-mentioned two kinds of tissue culture methods are not suitable for the cultivation of golden nails, adopt above-mentioned two kinds of methods to carry out the tissue culture of golden nails, its reproduction coefficient and survival rate are lower

Method used

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  • Medium and method for colorful-leaf euonymus japonicus tissue culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The tissue culture of embodiment 1 golden nail

[0047] 1. Growth environment

[0048] (1) A solid WPM medium is used, and the prepared medium is sterilized by high-pressure high-temperature steam, at 126° C. for 5 minutes.

[0049] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 48%, sterilized with ultraviolet light for 30min every 3d.

[0050] 2. Processing of Explants

[0051] (1) From May to June every year, young stems with buds that grow healthy and free from diseases and insect pests are used as explants.

[0052] (2) After the collection is completed, firstly remove the reserved petiole of the leaves, then wrap them in gauze and rinse them under running water for 2 hours.

[0053] (3) Clean the explants with a soft brush dipped in a solution containing 0.1% NaClO.

[0054] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of ...

Embodiment 2

[0083] The tissue culture of embodiment 2 golden nails

[0084] 1. Growth environment

[0085] (1) A solid WPM medium is used, and the prepared medium is sterilized by high-pressure high-temperature steam, at 126° C. for 5 minutes.

[0086] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 48%, sterilized with ultraviolet light for 30min every 3d.

[0087] 2. Processing of Explants

[0088] (1) From May to June every year, young stems with buds that grow healthy and free from diseases and insect pests are used as explants.

[0089] (2) After the collection is completed, firstly remove the reserved petiole of the leaves, then wrap them in gauze and rinse them under running water for 2 hours.

[0090] (3) Clean the explants with a soft brush dipped in a solution containing 0.1% NaClO.

[0091] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of...

Embodiment 3

[0120] The tissue culture of embodiment 3 golden nails

[0121] 1. Growth environment

[0122] (1) A solid WPM medium is used, and the prepared medium is sterilized by high-pressure high-temperature steam, at 126° C. for 5 minutes.

[0123] (2) Tissue culture room, light time 12h / d, light intensity 3500lx, culture temperature 25°C, relative humidity 48%, sterilized with ultraviolet light for 30min every 3d.

[0124] 2. Processing of Explants

[0125] (1) From May to June every year, young stems with buds that grow healthy and free from diseases and insect pests are used as explants.

[0126] (2) After the collection is completed, firstly remove the reserved petiole of the leaves, then wrap them in gauze and rinse them under running water for 2 hours.

[0127] (3) Clean the explants with a soft brush dipped in a solution containing 0.1% NaClO.

[0128] (4) Sterilize with 75% alcohol for 20 seconds and 0.1% HgCl for 5 minutes in the ultra-clean workbench. Note: The number of...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a medium and method for colorful-leaf euonymus japonicus tissue culture. The medium comprises a primary medium, a first subculture medium, a second subculture medium and a rooting medium. By means of the formula of the medium, the propagation coefficient of colorful-leaf euonymus japonicus can be remarkably improved, the propagation period is shortened, the survival rate is increased, and a more effective means is provided for industrial production of colorful-leaf euonymus japonicus.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of golden nails. Background technique [0002] Golden armor is a variant species of Euonymus japonicus cv. "Cu Zhi" in the Euonymus family Euonymus. Its leaves are golden on both sides and emerald green in the middle, hence the name. The golden beetle has a compact plant type and bright color, and inherits the characteristics of good cold resistance and resistance of the Hokkaido boxwood. It is a colorful non-deciduous plant that is lacking in the north. Golden beetle has strong uprightness, strong sprouting ability, high transplanting survival rate, and is easy to maintain. It can be cultivated into various shapes such as hedges, balls, and green walls. It is a garden tree species with great market potential. At present, the golden beetle is mainly propagated by grafting, which is time-consuming and laborious, and has a low reprod...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂何甜甜郭冠男孟伟芳
Owner 河南红枫生物高科股份有限公司
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