Embedding and slice making method of lateral buds at different node positions of sugarcane
A technology of lateral buds and sugarcane, applied in the field of embedding and slicing of plant tissue, to achieve the effect of increasing penetration, fast fixation speed, good scientific research guidance and practical significance
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Embodiment 1
[0047] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:
[0048] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;
[0049] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. Put in 4% ethylenediamine aqueous solution, let it stand at 4°C for ...
Embodiment 2
[0070] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:
[0071] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;
[0072] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. solution, put in 4% ethylenediamine aqueous solution, let it stand a...
Embodiment 3
[0093] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:
[0094] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;
[0095] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. Put in 4% ethylenediamine aqueous solution, let it stand at 4°C for ...
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