A method for embedding side buds at different nodes of sugarcane and making tablets

A lateral bud and sugarcane technology, applied in the field of plant tissue embedding and slice, to achieve the effect of increasing penetration, good effect and fast fixation

Active Publication Date: 2019-02-15
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems existing in the prior art, the present invention provides a method for embedding side buds at different nodes of sugarcane to make slices, which integrates the characteristics of the two embedding slice methods of paraffin slice embedding and resin permeation embedding, integrates the application, and learns from each other. To systematically solve the slice problem of sugarcane lateral bud cytology research

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  • A method for embedding side buds at different nodes of sugarcane and making tablets
  • A method for embedding side buds at different nodes of sugarcane and making tablets
  • A method for embedding side buds at different nodes of sugarcane and making tablets

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Embodiment 1

[0047] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:

[0048] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;

[0049] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. Put in 4% ethylenediamine aqueous solution, let it stand at 4°C for ...

Embodiment 2

[0070] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:

[0071] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;

[0072] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. solution, put in 4% ethylenediamine aqueous solution, let it stand a...

Embodiment 3

[0093] A method for embedding side buds at different nodes of sugarcane for tablet production, comprising the following steps:

[0094] (1) Sample collection and fixation: Cut the side buds on each stem section of freshly picked sugarcane with a double-sided blade and cut them longitudinally. For the buds corresponding to the segment of the first fully developed leaf starting from the slightly lower part, count as No. 1, and cut down in order according to the research needs , fix in separate bottles, and put about 2ml of 2.5% glutaraldehyde fixative solution prepared previously into each bottle;

[0095] (2) Softening: After about 4 hours in the previous step, choose to continue fixing the samples near the upper part (about No. 1 to No. 4 side buds), draw out the fixative solution for the remaining side buds, and wash the buffer three times with an interval of 10 minutes each time, and then absorb the buffer. Put in 4% ethylenediamine aqueous solution, let it stand at 4°C for ...

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Abstract

The invention relates to an embedding and slice making method of lateral buds at different node positions of sugarcane, and belongs to the technical field of the embedding and slicing of plant tissue. The method comprises the following steps of (1), taking and fixation of a sample, (2), softening, (3), dehydration, (4), displacement, (5), infiltration, (6), embedding, (7), trimming of a block, (8), slicing, (9), spreading of a slice, and adhesion of the slice, (10), dyeing, and the like. According to the method, the characteristics of two embedding and slicing methods of paraffin embedding and slicing and resin infiltrating and embedding are synthesized; the application is integrated; weak points are compensated by learning from strong points; the novel method is searched and developed to systematically solve the slicing problem of the cytological study of the lateral buds of the sugarcane; further, the made slice is intact and is almost not broken; slicing and embedding effects of giving consideration to a whole and a detail can be achieved.

Description

technical field [0001] The invention relates to a method for embedding lateral buds at different nodes of sugarcane and belongs to the technical field of plant tissue embedding and slicing. Background technique [0002] Plant tissue embedding and slicing technology is the most commonly used technology in biological slice technology. Because it can be cut into thin and continuous slices, plant anatomists mainly use it to observe the morphology of cell tissues and organs, and make permanent slices for preservation. The most commonly used embedding techniques are paraffin section embedding and resin infiltration embedding. The paraffin section embedding steps include sampling, fixation, dehydration, clearing, wax dipping, embedding, slicing, dewaxing, clearing, staining, etc., and the resin section embedding steps include sampling, fixing, post-fixation, dehydration, replacement, and resin infiltration. , embedding, sectioning and staining. Using the above two methods, there...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/36
CPCG01N1/36G01N2001/364
Inventor 李栋梁苏俊波孔冉罗炼芳沈炜棠
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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