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Molecular marker of rice blast resisting gene and application of molecular marker

A rice blast resistance gene and molecular marker technology, which is applied in the field of molecular genetics and breeding of crops, can solve the problems of limited resistance spectrum of disease resistance genes and lack of marker application system, and achieves low breeding efficiency, improved efficiency and clear selection goals. Effect

Active Publication Date: 2017-04-05
BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But on the other hand, many of the disease resistance genes that have been mapped or cloned do not have a broad spectrum of resistance, or lack an efficient marker application system

Method used

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  • Molecular marker of rice blast resisting gene and application of molecular marker
  • Molecular marker of rice blast resisting gene and application of molecular marker
  • Molecular marker of rice blast resisting gene and application of molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Genetic analysis of blast resistance traits of indica rice variety North Fujian

[0026] Minbei Indica is a local rice variety from Fujian Province. Use 193-1-1, 195-2-2, 318-2, 408, 501-3, CHE86, CHL1711, CHL1755, CHL1757, CHL1787, CHL1907, CHL1962, CHNOS, HYG99, IR16, from different rice regions at home and abroad KJ201, KOH, NY90, RB2, RB3, RB4, RB6, RB7, RB9, RB12, RB16, RB18, RB19, RB20, RB22, ROR1-1, ZY838, etc. 32 rice blast strains were inoculated with North Fujian indica. The resistance spectrum of northern indica to the inoculated strains reached 93.8%, indicating that northern Fujian indica has broad-spectrum resistance.

[0027] Use rice variety 02428 and Fujian North Indica to prepare F 2 Genetic population, inoculated with rice blast strain KJ201 F 2 group. To 627 F 2 The individual plant was investigated, and the number of resistant plants was 482 and the number of susceptible plants was 145, which accorded with 3:1 genetic segregation (Table 1), i...

Embodiment 2

[0030] Example 2 Bulked Segregant Analysis (BSA-seq) to identify the rice blast resistance gene locus of indica in northern Fujian

[0031] Based on the F of the above Minbei Indica×02428 2 The genetically isolated population was inoculated with Magnaporthe grisea KJ201, and 20 extremely disease-resistant plants and 20 extremely susceptible plants were randomly selected, as well as 10 Fujian indica plants, and 10 plants 02428. DNA was extracted separately, and the same amount was mixed into a disease-resistant pool. The susceptible pool, and the resistant parent pool, the susceptible parent pool. The DNA pool was sequenced using the second-generation high-throughput sequencing technology, and the average allelic frequency of the whole genome SNP (Avg. AF) was analyzed. The results showed that there is an AF higher than the threshold line in the 9.6-11.2 Mb interval of chromosome 6. Peak, there is a major disease resistance gene locus ( figure 1 ).

Embodiment 3

[0032] Example 3 Molecular marker location of rice blast resistance genes in indica in northern Fujian

[0033] In order to further determine the blast resistance gene locus of indica in northern Fujian, the present invention developed ID1002, ID1011, ID1027, ID1036 in the 10.0-10.8 Mb interval of chromosome 6 based on the second-generation sequencing results of two parents of indica and 02428 5 InDel molecular markers including, ID1081, and 1 dCAPS marker DC1045. Using these molecular markers to analyze the F of Minbei Indica ×02428 2 A population of 1051 susceptible individual plants were subjected to mapping analysis, and finally the rice blast resistance gene of indica rice in northern Fujian was located in the interval of about 89 kb between molecular markers ID1036 and DC1045 ( figure 2 ).

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Abstract

The invention discloses a molecular marker of a rice blast resisting gene and application of the molecular marker and belongs to the field of crop molecular genetics breeding science. The study means of phytopathology, molecular genetics and genomics are utilized, the rice blast resisting gene of the local rice variety of indica type rice in the northern Fujian province is located between the six chromosome molecular marker ID1036 and the six chromosome molecular marker DC1045. The molecular marker combination of ID1036 and DC1045 is utilized, plants, carrying the anti-disease gene, in the hybrid transform breeding group of the rice blast resisting variety of the indica type rice in the northern Fujian province can be efficiently screened, and the efficiency of breeding the rice blasting rice variety comprising the gene is improved.

Description

Technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and specifically relates to a molecular marker of rice blast resistance gene and its application. Background technique [0002] Rice is one of the most important food crops in the world, and rice blast is one of the most serious diseases that endanger rice production. Worldwide, the annual loss caused by rice blast is as high as 10-30% of rice production (Dean et al., 2005). Production practice shows that the most economical, effective and environmentally friendly strategy for the prevention and control of rice blast is to utilize rice disease-resistant genetic resources and to cultivate and utilize disease-resistant rice varieties. [0003] Since Japanese scientists took the lead in carrying out genetic research on rice blast resistance genes in the 1960s, scientists around the world have located hundreds of rice blast resistance genes. The cloning of rice blast resistance genes pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈松彪陈子强田大刚陈在杰王锋
Owner BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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