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Algal inhibition application of glyceroglycolipid compound and detection method of algal inhibition activity of glyceroglycolipid compound

A compound, glycerol sugar technology, applied in the fields of botanical equipment and methods, applications, biocides, etc., can solve the problems of the research on the algal inhibitory activity of Phaeocystis globosa, and the algae algae has not been seen yet, and achieve good algal inhibitory effect. , Easy to operate, reasonable design effect

Inactive Publication Date: 2017-04-19
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no study on the antialgal activity of these three glyceroglycolipid compounds on Heterocurvium akashiwo, Prorocentrum donghaiense and Phaeocystis globosa

Method used

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  • Algal inhibition application of glyceroglycolipid compound and detection method of algal inhibition activity of glyceroglycolipid compound
  • Algal inhibition application of glyceroglycolipid compound and detection method of algal inhibition activity of glyceroglycolipid compound
  • Algal inhibition application of glyceroglycolipid compound and detection method of algal inhibition activity of glyceroglycolipid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, with reference to Figure 1-Figure 4 , a kind of anti-algae application experiment of glyceroglycolipid compound:

[0027] 0.1 g of glyceroglycolipid compound was dissolved in 20 mL of methanol (analytical grade) to prepare a mother liquor with a concentration of 5 g / L. Pipette different volumes of mother liquor and add them to the red tide microalgae culture solution in the exponential growth phase, so that the final concentrations of glyceroglycolipids were 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL. mL (the maximum pipetting volume shall not exceed 1% of the culture system volume). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, potassium dichromate was set as the positive control group, that is, different volumes of potassium dichromate aqueous solutions were pipetted and added to the red...

Embodiment 2

[0028] Example 2, 2 Algae-inhibiting application experiment 2 of a glycerol glycolipid compound:

[0029] (1) Dissolve 0.05 g of glyceroglycolipid compound in 10 mL of methanol (analytical grade) to prepare a mother liquor with a concentration of 5 g / L;

[0030] (2) Pipette 1.6 µL, 8.0 µL, 40 µL and 200 µL of the mother solution, respectively, and add them to the red tide microalgae culture solution in the exponential growth phase, and the final volume of the culture system is 20 mL. The final concentrations of glyceroglycolipids were 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL (the maximum pipetting volume was 1% of the final volume of the culture system). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, 0.025 g of potassium dichromate (analytical grade) was dissolved in 5 mL of distilled water at a concentration o...

Embodiment 3

[0035] Embodiment 3, a kind of algae-inhibiting application experiment three of glycerol glycolipid compound:

[0036] (1) Dissolve 0.1 g of glyceroglycolipid compound in 20 mL of methanol (analytical grade) to prepare a mother liquor with a concentration of 5 g / L;

[0037] (2) Pipette 8 µL, 40 µL, 200 µL and 1000 µL of the mother liquor, respectively, and add them to the red tide microalgae culture solution in the exponential growth phase, and the final volume of the culture system is 100 mL. The final concentrations of glyceroglycolipids were 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL (the maximum pipetting volume was 1% of the final volume of the culture system). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, 0.05 g of potassium dichromate (analytical grade) was dissolved in 10 mL of distilled water at a conce...

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Abstract

The invention discloses algal inhibition application of a glyceroglycolipid compound. The algal inhibited by the glyceroglycolipid compound is red tide microalgae which is selected from Heterosigma akashiwo or Prorocentrum donghaiense or Phaeocystis globsa. The glyceroglycolipid compound is selected from 1-O-palmitoyl-3-O-BETA-D-pyran galactose glyceride, 1-O-octadecanoato-3-O-BETA-D-pyran galactose glyceride and 1-O-palmitoyl-2-O-oleate-3-O-BETA-D-pyran galactose glyceride. The invention further discloses a detection method of the algal inhibition activity of the glyceroglycolipid compound. New application of the glyceroglycolipid compound is achieved, the glyceroglycolipid compound is good in algal inhibition effect, and the application field of the compound is widened.

Description

technical field [0001] The invention belongs to the field of marine biochemical engineering, and specifically relates to the algae-inhibiting application of glyceroglycolipid compounds on red tide microalgae; the invention also discloses a method for measuring the algae-inhibiting activity of glyceroglycolipid compounds. Background technique [0002] Glyceroglycolipids mainly exist in the plant kingdom and microorganisms, and are the main components of plant chloroplast thylakoid membranes and bacterial plasma membranes, participate in cell recognition, signal transduction and other activities, and have anti-oxidation, anti-virus, anti-bacterial, anti-tumor, anti-inflammatory , anti-atherosclerosis and hemolysis and other biological activities. In recent years, it has been found that many macroalgae contain glyceroglycolipids. For example, kelp ( Laminaria japonica ), Kelp ( Ecklonia kurome ), brown algae Ectocarpus fasciculatus , Sargassum half leaf ( Sargassum he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P13/00
CPCA01N43/16
Inventor 孙颖颖郭赣林浦寅芳位义迎苏振霞
Owner HUAIHAI INST OF TECH
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