Cell bidirectional invasion monitoring method based on micro-fluidic chip

Abstract

The invention provides a cell bidirectional invasion monitoring method based on a micro-fluidic chip. According to the cell bidirectional invasion monitoring method, the micro-fluidic chip is adopted for monitoring the cell bidirectional invasion, the inducing or inhibiting effects of different cells or factors on the invasion of a target cell can be simultaneously observed and compared, and the inducing or inhibiting effects of one certain cell or factor on the invasion of different target cells also can be simultaneously observed. The micro-fluidic chip is mainly composed of three main channels and two collagen channels, wherein the three main channels used for cell culture horizontally communicate with the two collagen channels used for the cell migration observation; the left of the middle main channel (1) is connected with the left collagen channel (2), the right of the middle main channel (1) is connected with the right collagen channel (4), the left of the left collagen channel (2) is connected with the left main channel (3), and the right of the right collagen channel (4) is connected with the right main channel (5). The method has the feature of tracking the cell movement in real time, the accurate locating for the cell movement is realized, and meanwhile, the cell invasion capacity and selectivity can be observed and compared.

Description

technical field [0001] The invention relates to the technical field of applying microfluidic chip technology to real-time monitoring cell biology research, in particular to a microfluidic chip-based bidirectional cell invasion monitoring method. Background technique [0002] Since the development of cell biology, the main culture and research have relied on orifice plates and commercialized transwell chambers to focus on the observation of cell morphological changes, growth process, invasion and proliferation behaviors, etc. under the stimulation of single or multiple factors. Cell invasion generally starts from the cell's sensing of its microenvironmental stimuli. Microenvironmental stimuli activate a series of intracellular signal transduction pathways and gene transcription through cell surface receptors, and then through changes in cell polarity and cell adhesion. And de-adhesion, rearrangement of the cytoskeleton and other links, and finally complete the change of cell ...

AI Technical Summary

Problems solved by technology

At present, there have been preliminary studies on the use of microfluidic chips to monitor the process of cell invasion, but the existing microfluidic chip designs cannot achieve simultaneous observation and comparison. Parallel multi-group experiments in the research are likely to cause differences between different groups. Affect the accuracy of experimental data

The research and analysis that can simultaneously observe and compare the process of selective invasion and movement of cells from a two-dimensional plane to a three-dimensional matrix is ​​still in a blank stage

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