Method for enhancing plant insect resistance

A pest-resistant, plant-based technology, applied in botany equipment and methods, chemical equipment and methods, biochemical equipment and methods, etc., can solve problems such as reducing food safety, causing great harm to chemical pesticides, and increasing pest resistance, etc. Achieve the effects of reducing the number of eggs laid, increasing the density of epidermis and enhancing resistance

Inactive Publication Date: 2017-04-26
CHONGQING UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for enhancing the insect resistance of plants, aiming to solve the probl...

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  • Method for enhancing plant insect resistance
  • Method for enhancing plant insect resistance
  • Method for enhancing plant insect resistance

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Embodiment Construction

[0041] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0042] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0043] Such as figure 1 As shown, the method for enhancing plant insect resistance provided by the embodiments of the present invention may further comprise the steps:

[0044] S101: clone of tomato gene sequence Solyc11g069190.1;

[0045] S102: Construction of vector containing tomato gene sequence Solyc11g069190.1 and its functional identification;

[0046] S103: transformation of tomato with overexpression vector pLP35S-SIARF4;

[0047] S104: identification of transgenic lines,...

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Abstract

The invention discloses a method for enhancing plant insect resistance. The method for enhancing the plant insect resistance comprises the following steps that a tomato gene sequence Solycllg069190 0.1 is cloned; a carrier containing the tomato gene sequence Solycllg069190 0.1 is constructed, and the functions of the carrier are identified; over-expression of the carrier pLP35S-S1ARF4 is performed to convert tomatoes; transgenic lines are identified, and transgenic plants are obtained. By means of the means of genetic engineering, the auxin response gene S1ARF4 is subjected to over-expression in tomatoes, so that the epidermal hair density of the surfaces of the tomatoes is improved, and the plant insect resistance is enhanced; the laying performance of tetranychus cinnabarinus on the plants obtained after over-expression is remarkably lowered, and the number of laid eggs is decreased; meanwhile, under the identical condition, tetranychus cinnabarinus is more unwilling to move to leaves of the plants obtained after over-expression for feeding.

Description

technical field [0001] The invention belongs to the technical field of plant epidermal cell development, and in particular relates to a method for enhancing plant insect resistance. Background technique [0002] Epidermal hairs are developed from plant epidermal cells and are widely distributed in terrestrial plants. They are a specialized structure that grows in plant epidermal tissues. Studies have shown that plant epidermis can reduce the damage of herbivorous insects, ultraviolet radiation and excessive transpiration to plants, so it plays an important role in enhancing plant stress resistance. Cuticle hairs in plants can be divided into secretory cuticles and non-secretory cuticles, which can provide physical and chemical barriers for plants under stress conditions. [0003] To sum up, insect pests are very serious now, and chemical pesticides are very harmful, which increases the resistance of pests and reduces food safety. Contents of the invention [0004] The pu...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00C12N5/10
CPCC07K14/415C12N15/8203C12N15/8286
Inventor 邓伟苑瑜瑾龚泽皓张文法燕芳梅丽华
Owner CHONGQING UNIV
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