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Experiment method for proving connection relationship between NO and slit

A technology of gap junction and experimental method, which is applied in the biological field to achieve the effect of reducing the degree of damage and infiltration of inflammatory cells and protecting cerebral blood vessels

Inactive Publication Date: 2017-04-26
SHIHEZI UNIVERSITY
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Problems solved by technology

[0002] The regulation of the expression of inflammatory factors is a key step in the inflammatory response, which is closely related to autoimmune diseases and cancer. Nitric oxide (NO) plays an important role in the regulation of inflammatory factor expression, but most of the existing researches focus on the synthesis of NO. Regulatory effect on inflammatory factors, but there is no relevant report on whether NO protects target organs by regulating the expression of gap junctions in SHR peripheral blood T lymphocytes and regulating the release of inflammatory molecules

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  • Experiment method for proving connection relationship between NO and slit
  • Experiment method for proving connection relationship between NO and slit
  • Experiment method for proving connection relationship between NO and slit

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Embodiment Construction

[0022] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] 1 Selection of experimental animals: Normal WKY rats and spontaneously hypertensive rats (Spontaneously Hypertensive rats, SHR) were used in the experiment, regardless of sex.

[0024] 2 Grouping of experimental animals and preparation of models:

[0025] 12-week-old SHR and WKY rats were selected and randomly divided into three groups: WKY group, SHR group and SHR+NO intervention group, male or female, 10 rats in each group, and the SHR+NO group was treated with 10 μmol / kg·d-1 intraperitoneally Sodium nitroprusside was injected for 4 weeks, and the same volume of normal saline was injected intraperitoneally for 4 weeks in WKY group and SHR group.

[0026] Such as figure 1 As shown, an experimental method to prove the relationship between NO and gap junctions, including the following steps:

[0027]...

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Abstract

The invention discloses an experiment method for proving a connection relationship between NO and a slit. The method comprises the following steps: tail artery blood pressure monitoring; HE dyeing; and flow cytometry detection of rat peripheral blood T lymphocyte subgroup, and expression of CD4+, CD8+ and up-Cx40. A research result shows that the SHR microvascular endothelia are injured and have inflammatory cell infiltration, accompanied by rat peripheral blood T lymphocyte subgroup disorder and increased expression of the up-Cx40 in T lymphocytes, and NO intervention reduces the injured degree of the microvascular endothelia, corrects the disorder state of the peripheral blood T lymphocyte subgroup and reduces the expression of the up-Cx40 in T lymphocytes, so it is deducted that the NO possibly inhibits the slit between the SHR peripheral blood T lymphocytes to protect cerebral vessels.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an experimental method for proving the relationship between NO and gap junctions. Specifically, it explores whether NO protects target organs by regulating the expression of gap junctions in SHR peripheral blood T lymphocytes and regulating the release of inflammatory molecules. Background technique [0002] The regulation of the expression of inflammatory factors is a key step in the inflammatory response, which is closely related to autoimmune diseases and cancer. Nitric oxide (NO) plays an important role in the regulation of inflammatory factor expression, but most of the existing researches focus on the synthesis of NO. However, there is no relevant report on whether NO protects target organs by regulating the expression of gap junctions in SHR peripheral blood T lymphocytes and regulating the release of inflammatory molecules. Contents of the invention [0003] The purpose of th...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1429G01N2015/1006G01N15/01
Inventor 马克涛李新芝司军强
Owner SHIHEZI UNIVERSITY
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