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Method for qualitative and quantitative analysis of triazophos and kit used in the method

A technology of triazophos and a kit, which is applied in the field of food safety detection and immunoassay, can solve problems such as inapplicability of triazophos, and achieve the effects of ensuring sensitivity, improving sensitivity, and good reproducibility

Inactive Publication Date: 2017-04-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit is detected through a competitive reaction system, which overcomes the defect that the "sandwich" structure model adopted by the current biological barcode immunoassay method is basically not suitable for the detection of triazophos

Method used

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  • Method for qualitative and quantitative analysis of triazophos and kit used in the method
  • Method for qualitative and quantitative analysis of triazophos and kit used in the method
  • Method for qualitative and quantitative analysis of triazophos and kit used in the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation method of triazophos biological barcode immunoassay assay kit

[0050] Preparation of S11 and triazophos double-labeled colloidal gold probes

[0051] Gold Nanoparticle Synthesis Method

[0052] Add 98mL of deionized water to the siliconized beaker respectively, and then add 2mL of 50mM chloroauric acid stock solution respectively, so that the concentration of chloroauric acid is 1mM, stir on a magnetic stirrer at 1000rpm / min, heat at 250°C, continue to boil after the liquid boils Boil for 2 minutes; absorb 10mL of 38.8mM trisodium citrate, quickly add it to the beaker at one time, keep the stirring speed and heating temperature constant, and continue to boil for 6 minutes. It can be found that the color of the solution gradually changes from colorless to purple, and then into wine red. According to the different reducing doses of trisodium citrate added, it finally turns into different colors. After the liquid is cooled, deionized water is used to restore ...

Embodiment 2

[0076] Kit use method of the present invention

[0077] The specific operations of the triazophos colloidal gold composite probe prepared in the above implementation case 1 combined with the real-time quantitative PCR immunoassay assay kit are as follows:

[0078] S21. Take out the kit from the refrigerator at 4°C, and equilibrate at room temperature for 10 minutes;

[0079]S22, add each concentration triazophos standard substance (10.0ng L -1 -40 μg L -1 ) and 20 μL of each sample obtained after pretreatment, set up a repetition, then add 20 μL of magnetic nanoprobes to each well, fully oscillate and mix with a micro shaker, and then place the temperature at 37 ° C and a relative humidity of 70% constant temperature Incubate for 1 h in a constant humidity incubator;

[0080] S23, wash three times with PBST solution, dehybridize to obtain the biological barcode;

[0081] S24, put into fluorescent quantitative PCR to measure;

[0082] S25. Taking the concentration of the s...

experiment example 1

[0084] The methodological examination result of kit of the present invention

[0085] The kit prepared in Example 1 was identified according to the common verification procedures in this field, and the results are shown in Table 1.

[0086] The methodological testing result of test kit of the present invention of table 1

[0087]

[0088] The above results show that the accuracy, specificity, precision, sensitivity and stability of the "triazophos colloidal gold composite probe combined with real-time quantitative PCR immunoassay assay kit" are fully in line with the conditions.

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Abstract

The invention relates to the technical field of food safety detection and immunity analysis, and in particular relates to a method for qualitative and quantitative analysis of triazophos and a kit used in the method. The kit contains a Triazophos standard, a Triazophos nano probe and Triazophos double-labeled colloidal gold nanoparticles; the kit is combined with fluorescence quantitative PCR for simple, rapid, sensitive, specific and stable detection of Triazophos content in a to-be-tested sample, experimental reproducibility is good, and operation and production are easy.

Description

technical field [0001] The invention relates to the technical field of food safety detection and immunoassay, in particular to a method for qualitative and quantitative analysis of triazophos and a kit used in the method. Background technique [0002] With the gradual withdrawal of highly toxic pesticides from the market, triazophos has become the main pesticide to replace methamidophos, and is widely used in the control of rice stem borer in the middle and lower reaches of the Yangtze River. In recent years, its use has increased rapidly. While triazophos pesticide is entering the peak season of the market in my country, foreign countries have begun to restrict or prohibit the use and sales of triazophos pesticide. On November 28, 2004, the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China issued an emergency warning, saying that the European Union would officially ban the sale of 320 pesticides in the European Union ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543
CPCG01N33/577G01N33/54326G01N2430/10
Inventor 金茂俊王静杜鹏飞金芬佘永新邵华郑鹭飞王珊珊
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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