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Kit for measuring alpha fetoprotein

A technology for alpha-fetoprotein and alpha-fetoprotein antibody, which is applied in the fields of medicine and biochemistry, can solve the problems of low measurement accuracy and complicated operation of alpha-fetoprotein, and achieve the effects of accurate detection results and convenient detection process.

Inactive Publication Date: 2017-04-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problems of complex operation and low measurement accuracy in the detection process of alpha-fetoprotein in the prior art, and provide a kit for measuring rheumatoid factor

Method used

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  • Kit for measuring alpha fetoprotein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0086] Reagent R1:

[0087] HEPES buffer 150mmol / L

[0088] Sodium chloride 250 mmol / L

[0089] EDTA50mmol / L

[0090] Bovine Serum Albumin 44 g / L

[0091] Polyethylene glycol-6000 15 g / L

[0092] Tween-80 10 mL / L

[0093] Sodium azide 0.7 g / L

[0094] Anti-human rheumatoid factor antibody 1.0 g / L

[0095] Its solvent is purified water.

[0096] Reagent R2:

[0097] HEPES buffer 100 mmol / L

[0098] Sodium chloride 340 mmol / L

[0099] EDTA50mmol / L

[0100] Bovine Serum Albumin 44 g / L

[0101] Sodium azide 0.7 g / L

[0102] Latex-coated anti-human alpha-fetoprotein antibody 1.0 %

[0103] Its solvent is purified water.

Embodiment 2

[0105] Kit preparation and method of use

[0106] 1. Preparation of latex-coated anti-human alpha-fetoprotein antibody:

[0107] (a) Dilute latex particles with a particle size of 80nm to 3 g / L with 50 mmol / L MES buffer, add 0.5 mg EDAC to each mL solution, react at room temperature for 2 hours, and centrifuge at 20000 rpm Centrifuge at high speed for 30 minutes, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, and disperse by ultrasonic;

[0108] (b) Centrifuge the product of step (a) at 20,000 rpm for 30 minutes in a centrifuge, discard the supernatant, and suspend the precipitate in 50 mmol / L MES buffer, so that the final concentration of latex particles is 6g / L, Ultrasonic dispersion, add an equal volume of MES diluent containing anti-human alpha-fetoprotein antibody while stirring, mix and stir, react at room temperature for 2 hours, the final concentration of latex particles is 3.0 g / L;

[0109] (c) Centrifuge the product of step (b) at 20,000 rpm fo...

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Abstract

The invention relates to a kit for measuring alpha fetoprotein. The kit comprises double liquid components, namely, a reagent R1 and a reagent R2 which are independent of each other. According to the double liquid components, namely, the reagent R1 and the reagent R2 which are independent of each other, the reagent R1 is prepared from a 40-260 mmol / L HEPES buffer solution, 120-380 mmol / L sodium chloride, 20-80 mmol / L EDTA, 28-60 g / L bovine serum albumin, 5-25 g / L polyethylene glycol-6000, 4-16 mL / L Tween-80, 0.6-0.9 g / L sodium azide and 0.2-1.8 g / L human rheumatoid factor resisting antibodies, and purified water is adopted as a solvent of the reagent R1; the reagent R2 is prepared from a 50-150 mmol / L HEPES buffer solution, 180-500 mmol / L sodium chloride, 20-80 mmol / L EDTA, 28-60 g / L bovine serum albumin, 0.6-0.9 g / L sodium azide and 0.1%-2.0% of latex-wrapped human alpha fetoprotein resisting antibodies, and purified water is adopted as a solvent of the reagent R2.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring alpha-fetoprotein. Background technique [0002] Alpha-fetoprotein is a kind of glycoprotein. Under normal circumstances, this protein mainly comes from the liver cells of the embryo. About two weeks after the fetus is born, alpha-fetoprotein disappears from the blood. Therefore, the content of alpha-fetoprotein in normal human serum is less than 20 μg / L. [0003] Alpha-fetoprotein is mainly synthesized in the fetal liver, with a molecular weight of 69,000. At the 13th week of the fetus, AFP accounts for about 1 / 3 of the total plasma protein. It reached its peak at 30 weeks of gestation, and then gradually decreased. The plasma concentration at birth was about 1% of the peak, about 40mg / L, and it was close to the adult level at one year old. [0004] Alpha-fetoprotein in maternal amniotic fluid or maternal plasma AFP can be used for fetal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 蔡晓辉庄庆华吴铮
Owner ANHUI IPROCOM BIOTECH CO LTD
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