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Method for rapidly cultivating antrodia camphorata

A technology of rapid and sloping culture medium, applied in the field of edible fungus cultivation, can solve the problems of low yield of Antrodia camphorata, inability to obtain chemical components of wild Antrodia camphorata, long cultivation time, etc., achieve good social and economic benefits, alleviate the contradiction between supply and demand in the market, and alleviate the The effect of insufficient raw materials for pharmaceutical companies

Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the basswood cultivation method takes a long time to cultivate, and the cultivation cost is high, and the yield of Antrodia cinnamomea obtained is low. Although the solid-state cultivation method and the liquid fermentation method have a short cultivation time, they cannot obtain some unique chemical components of wild Antrodia camphorata, which cannot be obtained in the market. recognized

Method used

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  • Method for rapidly cultivating antrodia camphorata

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Embodiment 1

[0016] A method for quickly cultivating Antrodia camphorata, comprising the following steps:

[0017] 1) Preparation of Armillaria bacterial solution: Select young and robust wild Armillaria bacterial strands, take the tip, sterilize the surface with alcohol with a volume concentration of 70% for 5 minutes, and then remove the outer skin sheath under aseptic conditions. Cut the white mycelia into 1cm-long pieces, inoculate them on the slant culture medium, place a section in each tube, and cultivate them at 20°C for 20 days until the mycelium is covered with the slant; then cut the obtained mycelium into 1.0cm small pieces, Add glucose solution with a mass concentration of 1.0% at a weight-to-volume ratio of 1:3 (g / mL), homogenize at 1000 r / min for 10 min, and obtain Armillaria bacterium liquid;

[0018] 2) Armillaria inoculation: From October to November every year, saw a oak tree with a diameter of 10cm and grow it into a 30cm wood section. After stacking for 15 days, cut a ...

Embodiment 2

[0025] A method for quickly cultivating Antrodia camphorata, comprising the following steps:

[0026] 1) Preparation of Armillaria bacterial solution: Select young and robust wild Armillaria bacterial strands, take the tip, sterilize the surface with alcohol with a volume concentration of 70% for 6 minutes, and then remove the outer skin sheath under aseptic conditions. Cut the white mycelium into 1.2cm-long pieces, inoculate them on the slant culture medium, place a section in each tube, and cultivate at 22°C for 18 days until the mycelium is covered with the slant; then cut the obtained mycelium into 1.3cm small pieces , according to the weight to volume ratio of 1:2 (g / mL), add a glucose solution with a mass concentration of 1.2%, homogenize at 1000r / min for 10min, and obtain the Armillaria bacterium liquid;

[0027] 2) Armillaria inoculation: From October to November every year, saw a oak tree with a diameter of 12cm and grow it into a 30cm wood section. After stacking for...

Embodiment 3

[0034] A method for quickly cultivating Antrodia camphorata, comprising the following steps:

[0035] 1) Preparation of Armillaria bacterial solution: Select young and robust wild Armillaria bacterial strands, take the tip, sterilize the surface with alcohol with a volume concentration of 70% for 7 minutes, and then remove the outer skin sheath under aseptic conditions. Cut the white mycelia into 1.5cm-long pieces, inoculate them on the slant culture medium, place a section in each tube, and cultivate at 25°C for 15 days until the mycelia cover the slope; then cut the resulting mycelium into 1.5cm pieces , according to the weight to volume ratio of 1:2 (g / mL), add a glucose solution with a mass concentration of 1.5%, homogenize at 1000r / min for 10min, and obtain Armillaria bacterium liquid;

[0036] 2) Armillaria inoculation: From October to November every year, saw oak trees with a diameter of 15cm and grow into 30cm wood sections. After stacking for 20 days, cut a fish scale...

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Abstract

Then invention discloses a method for rapidly cultivating antrodia camphorata. The method includes the following steps that firstly, an oak segment is inoculated with armillaria mellea, nutrition of the oak segment is obtained depending on the growth and decomposition of the armillaria mellea, and then antrodia camphorata is inoculated, so that the antrodia camphorata obtains nutrition and grows fast through the armillaria mellea. The method not only protects the scarce resources of stout camphor trees, but also solves the bottleneck production problems, which resulting from malnutrition of antrodia camphorata, of slow growth and reproduction of antrodia camphorata hypha and long subculture cycle of the antrodia camphorata. The method can obtain antrodia camphorata fruiting body with comparatively high biomass and active ingredients that is similar to wild antrodia camphorata in a short period. The method can effectively alleviate not only the contradiction between the supply and the demand of the market but also the problem of raw-material shortage in pharmaceutical enterprises, and has good social and economic benefits.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, in particular to a method for quickly cultivating Antrodia camphorata. Background technique [0002] Antrodia Cinnamomea ( Antrodia camphorata ), also known as Cinnamomum camphorus, belongs to the perennial fungus of the genus Aphylloides, Polyporaceae, and Thinporus. , the favor of patients with diseases and the enthusiasm of the market. The artificial cultivation methods of Antrodia camphorata include camphor tree basswood cultivation method, solid-state cultivation method and liquid fermentation method. However, the basswood cultivation method takes a long time to cultivate, and the cultivation cost is high, and the yield of Antrodia cinnamomea obtained is low. Although the solid-state cultivation method and the liquid fermentation method have a short cultivation time, they cannot obtain some unique chemical components of wild Antrodia camphorata, which cannot be obtained in the mark...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14C12R1/645
CPCA01G18/00C12N1/14
Inventor 魏道智宁书菊
Owner FUJIAN AGRI & FORESTRY UNIV
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