Tissue isolation method for preparing litchi chinensis bacterium mother strain

Abstract

The invention discloses a tissue isolation method for preparing a litchi chinensis bacterium mother strain. The tissue isolation method comprises the following steps: selecting tender fruiting bodies of which the litchi chinensis bacterium mature degree is 40-45%, removing impurities, cleaning the tender fruiting bodies by using sterile water, then soaking the tender fruiting bodies in ethanol (75-90%) for 50-90s, flushing the tender fruiting bodies for 3 times by using the sterile water, then soaking the tender fruiting bodies in NaClO (3%), HgCl2 (0.1%) or 2,4-potassium sorbate (0.1%) for 1.5min, flushing the tender fruiting bodies for 3 times by using the sterile water, slightly cutting the tender fruiting bodies open by using a sterile scalpel, and selecting intermediate tissues of which the sizes are 0.25-0.35cm<3> for inoculation so as to obtain pure culture. The tissue isolation method disclosed by the invention can inhibit the growth of infectious microbes, and can keep the characteristics of a wild litchi chinensis bacterium mother strain to the maximum extent, the obtained mother strain has robust mycelia, is white in color and rapid in growth, and the method establishes an industrial technical foundation for artificial high-yield culture of litchi chinensis bacteria, and is great in economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a tissue separation method for preparing litchi fungus parent species. Background technique [0002] Litchi chinensis Bacterium (Litchi chinensis Bacterium) is an edible fungus that grows rapidly on moist termite nests in litchi forests in Guangdong. It is known as the "king of bacteria". Neng Guanyu is so named because the lychee fungus has unique flavors such as tender meat, fragrant taste, and fresh and sweet taste. In addition, it has very strict requirements on the growth environment. It only grows on the moist termite nests in the lychee forest and cannot be artificially cultivated. The growth cycle is only about 20 days, which makes the lychee fungus more precious. At present, the research on litchi fungus focuses on the screening of its mycelium culture conditions, and there is no report on the separation technology of its mother species, and the breeding and pro...

AI Technical Summary

Problems solved by technology

At present, the research on litchi fungus focuses on the screening of its mycelium culture conditions, and there is no report on the separation technology of its mother species, and the breeding and production of mother species is the key to the artificial cultivation of litchi fungus