[0025] The present invention provides a bactericidal and disease-preventing suspension seed coating agent. The bactericidal and disease-preventing suspension seed coating agent is composed of original drug A, original drug B and original drug C in a weight ratio of 0.5-4:4-8:10-14 Original drug A is fludioxonil technical; technical B is metalaxyl technical; technical C is azoxystrobin technical; preferably, the bactericidal and disease-preventing suspended species provided by the present invention The coating is prepared from the original drug A, the original drug B and the original drug C in a weight ratio of 1-3:5-8:11-13; further, the sterilization and disease prevention suspension seed coating provided by the present invention The agent is prepared from the original drug A, the original drug B, and the original drug C in a weight ratio of 1-3:6-7:11-13; further, the sterilization and disease prevention suspension seed coat provided by the present invention The agent is prepared from the original drug A, the original drug B and the original drug C in a weight ratio of 2-3:6-7:11-12; even, the sterilization and disease prevention suspension seed coating provided by the present invention is It is prepared from the original drug A, the original drug B and the original drug C in a weight ratio of 2:6-7:12; or the sterilization and disease prevention suspension seed coating provided by the present invention is made of the original drug A and the original drug B and original drug C are prepared in a weight ratio of 3:6:11-12.
[0026] The present invention provides the sterilization and disease prevention suspension seed coating agent at the same time, and also provides the application of the sterilization and disease prevention suspension seed coating agent in the prevention and treatment of stalk rot, fusarium wilt, root rot or standing blight of gramineous plants; Application in potato late blight or black mole disease; application in peanut white sclerosis or root rot; grasses, for example, can be common grasses such as wheat and corn.
[0027] The bactericidal and disease-preventing suspension seed coating provided by the present invention is a pharmaceutical composition based on azoxystrobin, fludioxonil and metalaxyl as the core. This pharmaceutical composition contains three effective ingredients and has a broad bactericidal spectrum. The use of active ingredients is low, resources are saved, and the production of resistance is effectively slowed down and governed. Suspended seed coating agent is an efficient and environmentally friendly formulation that complies with national policies, and is efficient, safe, and has a long duration, and is not subject to high temperature, sun and rain. The effect of leaching; the seed treatment agent containing the three active ingredients can not only effectively prevent soil-borne diseases, but also prevent some of the above-ground diseases, and the above-ground and underground unified prevention and control.
[0028] The present invention adopts A (fludioxonil technical), B (fine metalaxyl technical), C (azoxystrobin technical) and A+B+C in different proportions to treat potato mole and peanut root rot And the virulence of corn stalk basal rot bacteria were tested separately.
[0029] The determination test refers to "Pesticide Bioassay Technology" (edited by Chen Nianchun, published by Beijing Agricultural University Press) and "Pesticide Indoor Bioassay Test Guidelines NY/T1156.2-2006". This test uses the growth rate method (plate method). On the basis of the preliminary test, on the aseptic operation table, according to the test treatment, the pre-melted sterile medium is quantitatively added into the sterile conical flask, and 5ml of the drug solution is added to the container from low concentration to high concentration. In an Erlenmeyer flask with 45mL hot medium (PDA medium, 45-50°C), shake well and quickly pour it into a glass petri dish with a diameter of 90mm, and pour 10 mL of medicated medium into each petri dish. Set the water calmly and make a flat plate after cooling. Five replicates for each concentration. The blank control contains no active ingredients and only one active ingredient. The cultivated pathogenic bacteria, under aseptic conditions, use a sterile punch with a diameter of 4 mm to cut the bacterial cake from the edge of the colony, and inoculate the bacterial cake in the center of the medicated plate with an inoculator. The medium fits and the lid is covered. All operations above are performed aseptically on the clean bench. After treatment, place it in a constant temperature sterile incubator at 26±0.5°C, and take it out 6 days later. The cross method was used to measure the colony diameter (in millimeters) of each treatment, and calculate the average colony diameter, the regression equation of mycelial virulence and its EC 90 The synergy coefficient (SR value) or co-toxicity coefficient (CTC value) of the mixed agent.
[0030] Take the logarithm of the effective component concentration (μg/mL) of each treatment as the independent variable X, and the probability of the inhibition rate converted into the dependent variable Y, use the professional data statistical software DPS to process, calculate the virulence regression equation and the inhibitory concentration EC 90.
[0031] Calculate the synergy coefficient (SR value) of each medicine according to Wadley method, SR <0.5 is the antagonistic effect, 0.5≤SR<1.5 is the additive effect, and SR≥1.5 is the synergistic effect; the co-toxicity coefficient (CTC value) of each agent is calculated according to Sun Yunpei's method, CTC <80, the antagonistic effect between the components, CTC=80-120, the additive effect between the components, CTC> 120. There is synergy between the ingredients. The results are shown in Table 1, Table 2, and Table 3.
[0032] Table 1 Determination of the virulence of potato black mole (indoor formula screening)
[0033] Drug name Regression equation EC 90 (mg/L)
[0034] Remarks: 1. A means 97% fludioxonil technical, B means 87% metalaxyl technical, C means 95% azoxystrobin technical
[0035] It can be seen from Table 1 that different ratios of fludioxonil, metalaxyl and azoxystrobin have different levels of additive or synergistic effects on potato mole, especially when metalaxyl and fludioxon When the weight ratio of nitrile and azoxystrobin is 6:2:12, the SR value reaches 1.6, and the synergistic effect is the most obvious.
[0036] Table 2 Toxicity determination of peanut root rot bacteria (indoor formula screening)
[0037] Drug name Regression equation EC 90 (mg/L)
[0038] Remarks: 1. A means 97% fludioxonil technical, B means 87% metalaxyl technical, and C means 95% azoxystrobin technical.
[0039] It can be seen from Table 2 that fludioxonil, metalaxyl and azoxystrobin in different ratios have different degrees of additive or synergistic effects on peanut root rot fungi, especially when metalaxyl, metalaxyl, When the weight ratio of bacterazol and azoxystrobin is 6:2:12, the SR value reaches 1.5 and the synergistic effect is the most obvious.
[0040] Table 3 Determination of the virulence of corn stalk basal rot bacteria (indoor formula screening)
[0041]
[0042] It can be seen from Table 3 that fludioxonil, metalaxyl, and azoxystrobin have synergistic effects on corn stalk rot in different ratios, especially when metalaxyl, fludioxonil and When the weight ratio of azoxystrobin was 3.3:1.1:6.6, the co-toxicity coefficient reached 138.47, and the synergistic effect was the most significant.
[0043] At the same time, in order to effectively illustrate that the technical solution provided by the present invention is feasible, the present invention selects different implementation units to complete the efficacy test in the field with the same ratio. The results are shown in Table 4:
[0044] Table 4 The control effect of the three combinations on corn stalk base rot
[0045]
[0046] Remarks: The final concentration of the suspension seed coating agent used in the field efficacy test is 11%; the suspension seed coating agent is prepared from fludioxonil, metalaxyl and azoxystrobin in a weight ratio of 2:6:12. to make.
[0047] The field efficacy test results show (Table 4) that when the weight ratio of fludioxonil, metalaxyl and azoxystrobin is 2:6:12, it has obvious control effect on corn stalk base rot. 64.52-89.56%, the highest reached 89.56%, far higher than the control effect of the control agent, and no phytotoxicity to corn was found during the test, no effect on subsequent crops, and no effect on natural enemies and other beneficial organisms .
[0048] In addition, the inventor also conducted a large number of experiments on other crops for prevention and control, and obtained the following specific examples. The results are shown in Table 5.
[0049] Table 5 Control effects of different crops under different ratios
[0050]
[0051]
[0052]
[0053] It can be seen from Table 5 that metalaxyl, fludioxonil and azoxystrobin are compounded in different proportions (0.5-4:4-8:10-14) for corn wilt, wheat root rot, Wheat blight, potato late blight, potato black mole and peanut sclerotia have good control effects. When the ratio is 2-3:6:11-12, the control effect is the best, reaching 88% the above.
