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A method for reducing Maillard reaction in medium sterilization process

A Maillard reaction and culture medium sterilization technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of reducing the growth of Maillard reaction and fermentation inhibition, reducing the risk of contamination, building The effect of cost reduction and utilization improvement

Active Publication Date: 2019-10-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] Aiming at the lack of practical and effective reduction of Maillard reaction in the field of microbial fermentation in the prior art to the problem of microbial growth and fermentation inhibition, the present invention aims to solve the problem of using reducing sugars as carbon sources and amino compounds as nitrogen sources in microbial fermentation media. The Maillard reaction that commonly occurs in the high temperature and humidity sterilization process inhibits the growth and fermentation of microorganisms, and provides a method that uses dibasic acids and monohydric alcohols as Maillard reaction inhibitors, which can maintain the existing high temperature and humidity sterilization process and equipment and eliminate the need for medium separation and elimination, so as to meet the needs of the microbial fermentation field for economical and effective reduction of the Maillard reaction that inhibits microbial growth and fermentation

Method used

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  • A method for reducing Maillard reaction in medium sterilization process

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Embodiment 1

[0036] After preparing the medium in the fermenter, add azelaic acid at 100g / L medium, add methanol at a molar ratio of azelaic acid:methanol of 1:2, set the stirring speed of the fermenter at 100rpm, and feed steam to the fermenter. The temperature was maintained at 121°C for 30 minutes to achieve the sterilization effect. After sterilization, cool the temperature of the fermenter to 60°C and pass in sterile nitrogen for 15 minutes, then stop stirring, continue cooling, let stand for 20 minutes, and then discharge the organic phase and solid phase from the lower outlet of the fermenter. Restore the stirring speed to 100prm, add Lactobacillus casei seed liquid according to 10% of the medium volume, take a sample after fermenting at 42°C for 48 hours, and measure the color of the fermentation liquid with the platinum-cobalt colorimetric method, the smaller the value of the platinum-cobalt colorimetric method The lighter the color of the fermentation broth; use the absorbance at...

Embodiment 2

[0038] Compared with Example 1, in Example 2, sebacic acid was added at 50 g / L medium, and ethanol was added at a sebacic acid: ethanol molar ratio of 1:1.5, and the others were the same as in Example 1.

Embodiment 3

[0040] Compared with Example 1, Example 3 presses 150g / L medium undecanedibasic acid, presses undecanedibasic acid: n-propanol molar ratio is 1:1 and adds n-propanol, other and embodiment 1 same.

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Abstract

The invention provides a method for reducing Maillard reaction during a culture medium sterilization process. The method comprises the following steps: adding binary acid and monohydric alcohol into a prepared culture medium, carrying out stirring, carrying out sterilizing, carrying out cooling till the temperature is 50-100 DEG C, introducing sterile gas and enabling the sterile gas to pass through the culture medium, stopping stirring, and carrying out further cooling, wherein after layering of the system, an organic phase and a solid phase are separated out, and the culture medium is used for fermentation. With the adoption of the method provided by the invention, the Maillard reaction flux in the system can be effectively reduced, meanwhile, an intermediate product and a final product of the Maillard reaction are separated from the system through the extraction principle, compared with the discontinuous sterilization process, the one-step sterilization process provided by the invention has the advantages that the construction cost of the fermentation device is reduced by one half, the energy consumed during the sterilization process is reduced by one half, and thus the construction cost and operation cost of the device are obviously saved.

Description

technical field [0001] The invention relates to a method for optimizing and regulating the industrial process of microbial fermentation, in particular to a method for reducing the inhibitory effect of Maillard reaction on microbial growth and fermentation, and belongs to the technical field of microbial fermentation. Background technique [0002] In 1912, the French chemist Louis. Camilla Maillard accidentally discovered in the experiment that the carbonyl group of the carbonyl compound (reducing sugar) can react with the amino group in the amino compound (amino acid / polypeptide / protein). Melanin or quasi-melanin, the carbonyl ammonia reaction is also called Maillard reaction. [0003] After years of research by scholars from various countries, the currently recognized Maillard reaction mechanism can be divided into the following three stages: [0004] Initial stage [0005] Reducing sugars undergo condensation reactions with amino acids or proteins to generate organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07C12N1/00
Inventor 姚新武廖莎高大成张霖李晓姝师文静樊亚超孙启梅
Owner CHINA PETROLEUM & CHEM CORP