Ankara virus strain FAdV-HB and preparation and application of inactivated vaccine of ankara virus strain FAdV-HB

A technology of Ankara virus and inactivated vaccine, applied in the field of animal vaccine preparation, can solve the problem of strong pathogenicity of chickens, and achieve the effect of preventing Ankara disease, good safety and good stability

Inactive Publication Date: 2017-05-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Group I poultry adenoviruses are distributed worldwide and can infect a variety of poultry. Most of them are recessive infections in ducks and geese, but they are highly pathogenic to chickens.

Method used

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  • Ankara virus strain FAdV-HB and preparation and application of inactivated vaccine of ankara virus strain FAdV-HB
  • Ankara virus strain FAdV-HB and preparation and application of inactivated vaccine of ankara virus strain FAdV-HB
  • Ankara virus strain FAdV-HB and preparation and application of inactivated vaccine of ankara virus strain FAdV-HB

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 Ankara inactivated vaccine

[0029] 1. Isolation and identification of FAdV-HB strain

[0030] Since 2013, a poultry disease with a fatality rate of up to 80% has broken out in many broiler chicken farms in Hubei, Shandong, and Henan. Hepatitis symptoms. In 2015, the inventor successfully isolated the virus of this strain from the organs of dead chickens with severe hydropericardium and hepatitis in Macheng Chicken Farm, Hubei.

[0031] The specific operation steps are as follows:

[0032] 1.1 Virus isolation

[0033] Aseptically collect viscera (heart, liver) with obvious lesions from sick and dead chickens, cut them into pieces and homogenate, add sterilized PBS at a ratio of 1:4 (v / v), freeze and thaw repeatedly 3 times, centrifuge at 3000r / min for 30min, and take Add penicillin and streptomycin to the supernatant so that the final concentration is 10000 units / ml, and overnight at 4°C. Use a 0.22 μm filter to filter and sterilize,...

Embodiment 2

[0201] Example 2 Safety Test of Ankara Virus Disease Live Vaccine

[0202] 1 Vaccines and experimental animals

[0203] The Ankara inactivated vaccines produced by the inventors were used, and the batch numbers were 0101, 0102, and 0103 respectively. The chicken breed used for the trial was Bailaihang, provided by Shandong Jinan Spifry Poultry Technology Co., Ltd.

[0204] 2 Safety trials of vaccines

[0205] This embodiment has detected the safety of above 3 batches of laboratory vaccines, and immunized Bailaihang chicks, and used to detect the safety of the batch of laboratory vaccines to Bailaihang chicks, including the safety of a single-dose inoculation of the vaccine to Bailaihang chicks, single Safety of repeated dose vaccination and safety of single overdose vaccination.

[0206] 2.1 The safety of a single-dose inoculation to Bailaihang chicks

[0207] After the three batches of vaccines were inoculated with 1-3 day-old chicks (Bai Laihang) in a single dose, the ch...

Embodiment 3

[0218] Example 3 Vaccine efficacy test of Ankara inactivated vaccine

[0219] 1 Ankara inactivated vaccine and experimental animals

[0220] 3 batches of Ankara inactivated vaccines produced by the inventor, the batch numbers are 0101, 0102, 0103 respectively. Ankara virus antibody-negative 1-3 day-old healthy chicks were used as experimental animals, provided by Jinan Spifry Poultry Technology Co., Ltd.

[0221] 2 Experimental methods

[0222] 40 1-3 day old healthy chicks were randomly divided into 4 groups, 10 in each group, three batches of vaccines prepared in the laboratory (batch numbers 0101, 0102, 0103) were immunized to one group in each batch, and the vaccine was injected intramuscularly into each leg 0.3ml. The remaining group was the non-immune control under the same conditions.

[0223] 3. Immunoprotective test

[0224] 3.1 Detection of antibody level

[0225] After 14 days of immunization, 30 chickens in the immune antibody monitoring group and 10 non-immu...

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Abstract

The invention discloses an ankara virus strain FAdV-HB and a preparation and application of an inactivated vaccine of the ankara virus strain FAdV-HB. The strain is separated from a liver tissue sample of a diseased chicken house, and is proved to be serum-4 type fowl adenovirus after being subjected to sequencing, evolution analysis and regression experiments, that is, the strain is pathogen of the hydropericardium hepatitis syndrome. After plaque purification, the virus proliferates in a chicken hepatocellular carcinoma cell line (LMH), and a stable filial generation virus with high virus titer is kept for the preparation of the inactivated vaccine. The animal experiment proves that the inactivated vaccine reaches 100% of protection rate over the ankara virus, high content of antibody is produced, the holding time of the antibody is long, the ankara disease is effectively prevented, and the huge economic losses of the breeding industry caused by the infection of the ankara virus are reduced.

Description

technical field [0001] The invention relates to the technical field of animal vaccine preparation, in particular to the preparation and application of an Ankara virus strain FAdV-HB and an inactivated vaccine thereof. Background technique [0002] Fowl adenovirus (Fowl adenovirus) is divided into three groups according to different group-specific antigens: group I, group II and group III. Group I adenoviruses include traditional adenovirus (Fowl adenovirus, FAV) and other poultry isolates, a total of 12 serotypes, the representative strain is chicken embryo lethal orphan virus (chicken embryo lethal orphan, CELO). Group II avian adenoviruses include turkey hemorrhagic enteritis virus (HEV), pheasant marble spleen virus, and chicken large spleen virus, which are not antigenically related to group I avian adenoviruses (Domermuth et al., 1980). Group III avian adenoviruses are related to egg drop syndrome (EDS). They can be isolated from chickens, chickens, and geese, and thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/235A61P31/20C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552C12N7/00C12N2710/10221C12N2710/10234
Inventor 金梅林马季杨影张丹康超李淑云邹忠林显孙小美
Owner HUAZHONG AGRI UNIV
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