Unlock instant, AI-driven research and patent intelligence for your innovation.

NtHMA2 gene mutant and application

A technology of mutants and genes, applied in the field of NtHMA2 gene mutants and applications

Active Publication Date: 2017-05-10
YUNNAN ACAD OF TOBACCO AGRI SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxicity of nickel and its salts is low, but because of its biochemical activity, it can activate or inhibit a series of enzymes (arginase, carboxylase, acid phosphatase and decarboxylase) to exert its toxicity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • NtHMA2 gene mutant and application
  • NtHMA2 gene mutant and application
  • NtHMA2 gene mutant and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The present invention will be further described below in conjunction with the embodiments and accompanying drawings, but the present invention is not limited in any way. Any transformation or replacement based on the teaching of the present invention belongs to the protection scope of the present invention.

[0035] NtHMA2 gene mutant of the present invention, and wild-type tobacco NtHMA2 Compared with the nucleotide sequence shown in SEQ ID No: 1 in the sequence table, the gene has a mutant containing NtHMA2 The C at position 520 of the gene sequence was mutated into a T, forming a stop codon and causing the gene to terminate prematurely.

[0036] NtHMA2 The amino acid sequence encoded by the gene is shown in SEQ ID No: 2;

[0037] NtHMA2 The nucleotide sequence of the gene mutant is shown in SEQ ID No: 3;

[0038] NtHMA2 The amino acid sequence encoded by the gene mutant is shown in SEQ ID No: 4;

[0039] said NtHMA2 The preparation method of the EMS mutant of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an NtHMA2 gene mutant. The mutation of one piece of nucleotide happens in an NtHMA2 gene, a non-mutation NtHMA2 sequence comprises a SEQ ID NO:1 nucleotide sequence, wherein C mutation on a 520 site is T, a termination codon is formed, and the gene is terminated in advance. Compared with a tobacco leaf which contains a SEQ ID NO:1 sequence, the tobacco which contains a mutation sequence is characterized in that a cadmium content is lowered by about 40%, the contents of zinc, iron, lead, chromium, nickel and the like are lowered by about 30%, and an arsenic content is lowered by about 10%.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a NtHMA2 Gene mutants and applications. Background technique [0002] Cadmium (Cd), arsenic (As), chromium (Cr), mercury (Hg) and lead (Pb) are recognized as heavy metal elements that are relatively harmful to plants and humans. Plant essential trace elements zinc (Zn), nickel ( Ni), copper (Cu), etc. will also cause harm if excessive. Therefore, research on crop heavy metal pollution and its reduction methods has attracted more and more attention from researchers. tobacco( Nicotiana tabacum ) is an important economic crop and is an annual herb of Solanaceae. In recent years, the research on heavy metals in tobacco has gradually become a hot spot in tobacco harm reduction research. [0003] Heavy metals can have adverse effects on tobacco quality. Nicotine content is an important index to measure the quality of tobacco leaves. Increasing nicotine wit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29
Inventor 王丙武高玉龙李文正宋中邦焦芳婵吴玉萍李永平
Owner YUNNAN ACAD OF TOBACCO AGRI SCI