Aptamer test strip adopting dual competitive method and application of aptamer detection strip

Abstract

The invention relates to the field of biomedical detection, in particular to an aptamer test strip adopting a dual competitive method and an application of the aptamer test strip. The test strip comprises a sample absorption pad, a marker pad, a reaction film, a water absorption pad and a bottom plate, wherein the marker pad is coated with a detecting probe, and the detecting probe is the aptamer marked with a detection target of an indicator for displaying signal strength; a detecting area and a quality inspection area are arranged on the reaction film; the detecting area is fixedly coated with a target antigen; the quality inspection area is fixedly coated with a quality control probe; the nucleic acid sequence of the quality control probe is complementary to the nucleic acid sequence of the aptamer.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a double competition method aptamer detection test strip and its application. Background technique [0002] Nucleic acid aptamer (Aptamer) is a single-stranded oligonucleotide, mostly single-stranded DNA, with a length between 10 and 100 bp. It is usually passed in vitro from a random sequence oligonucleotide library using a specific target Obtained through screening, as a new type of biorecognition molecule, it can specifically bind to target substances with high affinity. At present, there are hundreds of aptamer targets reported in the literature, and the established aptamer-based detection methods mainly include hundreds of methods such as colorimetry, fluorescence, and electrochemical methods, but so far, aptamer-based There are almost no commercial detection products for the body. [0003] Colloidal gold immunochromatography test strip technology is currently one of th...

AI Technical Summary

Problems solved by technology

Since the aptamer chain is generally relatively long, the affinity between the aptamer and the complementary chain is often greater than the affinity between the aptamer and the target, resulting in the inability to detect; the current literature solution is to truncate the complementary chain, and only partially The complementary chain is immobilized on the detection line, thereby reducing the affinity of the complementary chain and the aptamer, but this method requires complicated exploration, and when the length of the complementary chain is too short, the aptamer cannot hybridize with the complementary chain, which also leads to failure of detection

Images