Compositions and methods for identifying and selecting maize plants with resistance to northern leaf blight
A technology for leaf blight resistance and corn, applied in botany equipment and methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of reducing leaf surface area and affecting grain yield, etc.
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Embodiment 1
[0168] Phenotypes of Northern Leaf Blight Infection
[0169] Maize plants can be rated for northern leaf blight (NLB) on a scale of 1 (highly susceptible) to 9 (highly resistant), where a score of 1-3 indicates "susceptible", a score of 4-6 indicates "intermediate", and a score of 7 A score of -9 indicates "resistance". figure 1 The score chart in can be used as a guide, where the focus is on damage on the ear. Lesions can be verified to be caused by northern leaf blight infection by checking that the lesions are cigar or boat shaped with smooth sides and / or by sending samples to a diagnostic laboratory to confirm the identity of the pathogen.
[0170]Two to four weeks after flowering, scores can be obtained for some known susceptible lines and then compared to their historical scores. If a known susceptible line ranks at least two points higher than its historical score, the scoring of that line in the test group can be delayed, allowing the disease to progress to a stan...
Embodiment 2
[0173] association mapping analysis
[0174] Identification of maize genetic markers associated with resistance to northern leaf blight using an association mapping strategy.
[0175] use SNP genotyping (1536-plex assay) analyzes a pool of maize lines. SNP variants were used to generate specific haplotypes across inbred lines for genomic regions. This data was used to identify associations between alleles and resistance to northern leaf blight at the genomic level.
[0176] Incorporate resistance scores and genotype information into association mapping analysis. Structure-based association analysis was performed using standard association mapping methods, with marker data used to control for population structure. Two chromosome 5 markers, PHM16750 and PHM15741, were significantly associated with the northern leaf blight resistance trait in non-panic stem subpopulations. In addition, three chromosome 5 markers, PHM16854, PHM3870, and PHM14018, were significantly associa...
Embodiment 3
[0186] QTL mapping using double haploid breeding populations
[0187] QTL interval mapping analysis was performed to identify chromosomal intervals and markers associated with resistance to northern leaf blight using the 186 double haploid population generated from crosses between PHBNB and PHFHH. Line PHBNB was more resistant to northern leaf blight infection than line PHFHH. Double haploid lines resulting from crosses were phenotyped under natural northern leaf blight infection in a single growing season and in two locations. Maize double haploid progeny were genotyped using a panel of 768 SNPs distributed throughout the maize genome.
[0188] A significant peak was identified on chromosome 5 between 90 and 100 cM on the internally derived monomeiotic gene map, indicating that this region harbors one or more QTLs associated with resistance to northern leaf blight. Multiple markers showed associations with phenotypes at a confidence level of p<0.05 using interval mapping ...
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