Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method

An anti-nutritional factor and antigenic protein technology is applied in the field of efficient digestion of animal breeding anti-nutritional factors and antigenic proteins in soybean meal by acetic acid method to achieve the effect of improving nutritional quality and utilization value and efficient digestion

Pending Publication Date: 2017-05-17
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Purpose of the invention: Aiming at the problem of multiple anti-nutritional factors and antigenic proteins in soybean meal, the study found that using a certain concentration of acetic acid to quickly process soybean meal under heating conditions can efficiently digest the anti-nutritional factors and antigens in soybean meal that affect animal breeding Protein, in order to achieve the purpose of improving the nutritional quality and utilization value of soybean meal quickly and easily

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 40g of soybean meal (dry weight) into a 1000mL stainless steel tank reactor, add 10% acetic acid at a solid-to-liquid ratio of 1:5, place in an electric heating constant temperature oil tank, and catalyze at 120°C for 30min. After drying, the soybean meal product catalyzed by acetic acid is obtained. It was found by SDS-PAGE that glycinin and β-conglycinin were completely digested; at the same time, trypsin inhibitor was reduced from 9.504mg / g to 0.341mg / g, which was 96.41%.

Embodiment 2

[0020] Add 1 kg of soybean meal (dry weight) into a 15L rotary cooker, add 10% acetic acid at a solid-to-liquid ratio of 1:5, and catalyze at 150° C. for 10 minutes to obtain the treated material. Evaporate and condense the acetic acid-water azeotrope contained in the processed material by vacuum evaporation, recover more than 92% of acetic acid, add an appropriate amount of powdered calcium carbonate to neutralize the material to a pH of 4.5-6.0, and dry it to obtain Digested soybean meal products. It was found by SDS-PAGE that glycinin and β-conglycinin were completely digested; at the same time, trypsin inhibitor was reduced from 9.504mg / g to 0.309mg / g, which was 96.75%.

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Abstract

The invention discloses a method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by an acetic acid method. The soybean meal is the most widely-used plant protein raw material in the feed industry, but a variety of antinutritional factors and antigen proteins exist in the soybean meal, and must be destroyed by certain processing before the soybean meal is used for breeding animals. According to the method, acetic acid with a certain concentration is used for rapidly treating the soybean meal under a heating condition, so that two typical antigen proteins, namely glycinin and beta-conglycinin, can be completely digested, the content of trypsin inhibiting factors is reduced from 9.5mg / g to 0.3mg / g, and the digestion rate reaches 96% or above. By the method, an effective, simple, convenient and rapid production technology is provided for significantly increasing the nutritional value of the soybean meal and improving the breeding efficiency.

Description

[0001] 1. Technical field [0002] The invention relates to the fields of feed production, animal breeding and animal nutrition, in particular to a method for efficiently digesting animal breeding anti-nutritional factors and antigenic proteins in soybean meal by acetic acid method. [0003] 2. Background technology [0004] Soybean meal is a by-product of soybean oil extracted from soybeans. It is rich in various nutrients, including 40% to 50% protein, 1% to 2% fat, 10% to 15% carbohydrates, and a variety of nutrients. Minerals, vitamins, etc., with relatively complete and balanced nutritional components, are currently the most widely used plant-based protein feed ingredients. However, there are many anti-nutritional factors in soybean meal, such as trypsin inhibitor, antigenic protein and so on. The most important anti-nutritional factor, trypsin inhibitor, inhibits certain digestive enzymes in animals, and complexes with nutrients to form indigestible components, which red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20A23K10/37
CPCY02P60/87
Inventor 徐勇黄露余世袁
Owner NANJING FORESTRY UNIV
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