Device for generating concentration gradient and method for generating concentration gradient in one step

Abstract

The application discloses a concentration gradient generating device and a method for generating the concentration gradient in one step. The concentration gradient generation device includes first and second microchannel networks arranged vertically, the first and second microchannel networks respectively include several first and second concentration generation channels, and one end of each first concentration generation channel is All are communicated with the first sampling port of the first microchannel network, and one end of each second concentration generating channel is connected with the second sampling port of the second microchannel network, and the first and second sampling ports are respectively used to inject the 1. The second fluid, and the other end of each first concentration generating channel and the other end of a corresponding second concentration generating channel are simultaneously communicated with a connecting channel, so that the first and second fluids can flow in the connecting channel converging, while each connecting channel also communicates with a mixing channel. The concentration gradient generation device of the present application has the advantages of simple structure, good stability, and easy manufacture, and can form samples with various target concentrations in one step.

Description

technical field [0001] The application relates to a concentration gradient generating device, in particular to a device and method capable of generating a concentration gradient in one step, belonging to the technical fields of biology and analytical chemistry. Background technique [0002] The preparation of concentration gradient solutions in biochemical experiments is one of the most basic operations. In cell experiments and drug screening experiments, the behavior and toxicity of different concentrations of biomolecules and drugs to cells are quite different. Therefore, when investigating the effects of different concentrations of biomolecules and drug doses on cell behavior and toxicity, it is necessary to prepare a set of target solutions with gradient distributions. However, the traditional concentration gradient preparation method requires accurate calculation, weighing and multiple solution mixing, requires many chemical containers, and the operation is cumbersome, ...

AI Technical Summary

Problems solved by technology

However, this kind of concentration gradient generating device has a complex microchannel network structure, which requires a long straight channel or a complicated micromixer to achieve each separation and mixing process of the fluid, and its structure complexity and complexity increase with the increase of the target concentration. The number of microchannels has also increased, resulting in a complex structure, inconvenient manufacture, and large redundancy of the concentration gradient generation device; the concentration gradient generation device based on molecular diffusion is simple in structure, easy to operate, and easy to manufacture and process. The principle of diffusion, the concentration gradient generated by this chip will change with time, it is difficult to obtain a stable concentration gradient, and the flow rate, molecular size, liquid viscosity and diffusion distance all have a large influence on the generation of concentration gradient influences

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