Biological synthesis method of (R)-o-chloromandelic acid

A technology of o-chloromandelic acid and o-chloromandelonitrile, applied in the field of bioengineering, can solve the problems of high price of chiral resolving agent and low atom economy, achieve mild reaction conditions, no pollution in reaction conditions, and simple process route Effect

Inactive Publication Date: 2017-05-17
枣庄市杰诺生物酶有限公司 +1
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  • Abstract
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Problems solved by technology

However, because the chiral resolving agent required for optical isomer resolution is expensive, and by-product enantiomers will be obtained after resolution, atom economy is not high

Method used

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  • Biological synthesis method of (R)-o-chloromandelic acid
  • Biological synthesis method of (R)-o-chloromandelic acid

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Embodiment

[0049] The present embodiment discloses a method for biosynthesizing (R)-o-chloromandelic acid, which comprises the following steps:

[0050] (1) Take out the E.coli engineered strains preserved at -80°C, inoculate them on LB agar plates, and then culture them overnight at 37°C;

[0051] (2), picking the monoclonal colony on the LB agar plate, and inoculating it in the LB liquid medium, then culturing overnight at 37° C. at 200 rpm;

[0052] (3) Take an appropriate amount of bacterial liquid from the LB liquid medium after overnight cultivation, inoculate it in the LB liquid medium, cultivate it at 37° C. at 200 rpm for 8 hours, then inoculate it in the fermentation medium (as shown in Table 1), and inoculate Cultured at 37℃ to OD 600 to 20, then add an appropriate amount of IPTG, lower the temperature to 30°C and continue to cultivate for 16 hours, collect the bacteria by centrifugation (take the precipitate), and use NaH with a pH of 8.0 2 PO 4 / Na 2 HPO 4 The buffer so...

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Abstract

The invention provides a biological synthesis method of (R)-o-chloromandelic acid. The biological synthesis method comprises the following steps: feeding o-chloromandelic nitrile into a converting solution, conducting reaction for 8-12h under the conditions that the pH is 7.0-9.0 and the temperature is 20-40 DEG C, stopping feeding, and continuing conducting reaction for 1-4h to obtain the (R)-o-chloromandelic acid, wherein the converting solution comprises MG nitrilase-containing E.coli engineering bacteria. The method, combining use of the MG nitrilase and feeding of the o-chloromandelic nitrile, is used for preparing the (R)-o-chloromandelic acid, so that the process route is simple, reaction conditions are mild, pollution is free, the accumulated concentration of the (R)-o-chloromandelic acid during a single-batch reaction is as high as 600mM, and the ee value is greater than 98%; therefore, the biological synthesis method has a good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for biologically synthesizing (R)-o-chloromandelic acid. Background technique [0002] (R)-o-chloromandelic acid is an important chiral drug intermediate, which is mainly used in the synthesis of the anti-platelet aggregation drug clopidogrel. At present, the consumption of (R)‐o-chloromandelic acid is increasing day by day at home and abroad, and the market demand is increasing day by day. [0003] The synthesis methods of (R)-o-chloromandelic acid mainly include asymmetric synthesis method and optical isomer resolution method, but the optical isomer resolution method is the main method. Mandelic acid to obtain. However, because the chiral resolving agent required by the optical isomer resolution method is expensive, and by-product enantiomers will be obtained after the resolution, the atom economy is not high. Contents of the invention [0004] Main object of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12R1/19
CPCC12P7/42
Inventor 魏东芝许向阳宋在伟王华磊
Owner 枣庄市杰诺生物酶有限公司
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