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Method for preparing chenodeoxycholic acid by lithocholic acidase catalysis

A technique of chenodeoxycholic acid and lithocholic acid enzyme, which is applied in the field of chemical substance preparation, and can solve problems such as development limitation of chenodeoxycholic acid

Active Publication Date: 2017-08-22
眉山市新功生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current main idea for preparing chenodeoxycholic acid is to extract it from duck bile and goose bile, which limits the development of chenodeoxycholic acid.

Method used

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  • Method for preparing chenodeoxycholic acid by lithocholic acidase catalysis

Examples

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Effect test

Embodiment 1

[0028] A method for preparing chenodeoxycholic acid by a lithocholic enzyme catalyzed method, comprising the steps of:

[0029] Step 1. Enzyme Extraction

[0030] 1.1 Collect the cecum part of the domestic goose just slaughtered, scrape the intestinal mucosa with wood chips, put the obtained goose cecal mucosa liquid into a centrifuge tube, add a small amount of distilled water to dilute and shake well, centrifuge, and draw the supernatant.

[0031] 1.2 Under a sterile environment, inoculate the supernatant obtained above on a liquid LB medium containing 0.1% lithocholic acid; avoid light, control the temperature at 15-25°C, and grow colonies on the medium 3-4 days after inoculation Re-inoculate on the liquid LB medium containing 1% lithocholic acid, avoid light, control the temperature at 15-25°C, and cultivate for 3-4 days to obtain the required strains, store them on the slant medium of the test tube at 0-4°C, stand-by.

[0032] The described liquid LB culture medium that...

Embodiment 2

[0043] A method for preparing chenodeoxycholic acid by a lithocholic enzyme catalyzed method, comprising the steps of:

[0044] Step 1. Enzyme Extraction

[0045] 1.1 Collect the cecum part of the domestic goose just slaughtered, scrape the intestinal mucosa with wood chips, put the obtained goose cecal mucosa liquid into a centrifuge tube, add a small amount of distilled water to dilute and shake well, centrifuge, and draw the supernatant.

[0046] 1.2 Under a sterile environment, inoculate the supernatant obtained above on a liquid LB medium containing 0.1% lithocholic acid; avoid light, control the temperature at 15-25°C, and grow colonies on the medium 3-4 days after inoculation Re-inoculate on the liquid LB medium containing 1% lithocholic acid, avoid light, control the temperature at 15-25°C, and cultivate for 3-4 days to obtain the required strains, store them on the slant medium of the test tube at 0-4°C, stand-by.

[0047]The described liquid LB culture medium that ...

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Abstract

The invention discloses a method for preparing CDCA (chenodeoxycholic acid) with lithocholic acid enzyme catalysis. The method comprises the following steps: step 1, enzyme extraction: goose cecum mucous membrane liquid is obtained from goose cecum and centrifuged, required bacteria are obtained through inoculated culture, dilution is performed with distilled water, a diluent is subjected to pH regulation, mashed and centrifuged, and a supernatant, namely, a 7alpha-hydroxylase liquid, is drawn and stored at subzero 20 DEG C for standby application; step 2, catalytic conversion: lithocholic acid is catalyzed by the 7alpha-hydroxylase liquid, and CDCA is obtained. 7alpha-hydroxylase is obtained by culturing the goose cecum mucus, and raw materials are widely sourced and low in cost. The method overcomes the defect of the conventional idea for extracting CDCA from duck's bile and provides a new idea for synthesizing CDCA, and rejected CDCA products containing high lithocholic acid are transformed into qualified CDCA products.

Description

technical field [0001] The invention relates to a preparation method of a chemical substance, in particular to a method for preparing chenodeoxycholic acid by catalyzing a lithocholic acid enzyme. Background technique [0002] The chemical name of chenodeoxycholic acid (CDCA) is 3α, 7α-dihydroxy-5β-cholic acid, and its molecular formula is C 24 h 40 o 4 , molecular weight 392.58. It was first extracted from goose bile in 1848. Chenodeoxycholic acid has the ability to dissolve gallstones and other pharmaceutical functions. Since the 1970s, it has been clinically used as a drug for the treatment of gallstones and other hepatobiliary diseases. Recent studies have found that ursodeoxycholic acid, an isomer of chenodeoxycholic acid, has a better clinical effect in dissolving gallstones, and has no side effects. Therefore, ursodeoxycholic acid has gradually replaced chenodeoxycholic acid in the clinical application of gallstone-dissolving drugs. [0003] Natural ursodeoxycho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/06C12N9/02
CPCC12N9/0071C12P33/06
Inventor 梁万超蒋先曲
Owner 眉山市新功生物科技有限公司