Preparation method of chitosan oligosaccharide -based polyelectrolyte styptic powder

A polyelectrolyte and chitosan oligosaccharide technology, applied in bandages, absorbent pads, medical science, etc., can solve the problem of hemostatic drugs that cannot meet the requirements, achieve good biocompatibility and biodegradability, small particles, and fast absorption Effect

Inactive Publication Date: 2017-05-24
INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a preparation method of polyelectrolyte hemostatic powder based on chitosan oligosaccharide, the obtained polyelectrolyte hemostatic powder exhibits excellent hemostatic function, and solves the problems that existing conventional hemostatic methods and hemostatic drugs cannot meet the requirements.

Method used

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Examples

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Effect test

preparation example Construction

[0027]In a specific embodiment, the preparation method of the chitosan oligosaccharide-based polyelectrolyte hemostatic powder of the present invention first dissolves chitosan oligosaccharide and biocompatible anionic polyelectrolyte or amphoteric polyelectrolyte in deionized water. By reducing the pH value of the solution, the amino groups of chitosan molecules are protonated and positively charged, and interact with anionic polyelectrolytes or amphoteric polyelectrolytes to form complexes through Coulomb force. The polyelectrolyte complex is dried and pulverized to obtain chitosan polyelectrolyte hemostatic powder. The specific steps are:

[0028] 1) Prepare an aqueous solution of polyelectrolyte mixture containing chitosan oligosaccharide and anionic polyelectrolyte or amphoteric polyelectrolyte. Anionic polyelectrolytes or amphoteric polyelectrolytes are biocompatible polyelectrolytes containing -COO - 、-SO 3- 、-O-CS 2- ,-O-PO 3 2- Natural, semi-synthetic or synthet...

Embodiment 1

[0033] 10 grams of chitosan oligosaccharide, 13 grams of sodium polyacrylate and 1 g of gluconolactone were dissolved in 1 liter of deionized water, and incubated at 50° C. for 10 hours. After the polyelectrolyte complex was dried in a blast dryer at 50° C. for 60 hours, it was ball-milled for 1 hour with a ball mill to obtain chitosan polyelectrolyte hemostatic powder. The hemostatic powder has good biocompatibility and antibacterial property, and exhibits excellent hemostatic function. The measurement of coagulation index in vitro in this embodiment shows that the coagulation index in vitro is <41%; the hemostatic effect test result of the rabbit ear artery bleeding model shows that the hemostatic time is <97 seconds.

Embodiment 2

[0035] 10 grams of chitosan oligosaccharides, 10 grams of hyaluronic acid and 1 g of gluconolactone were dissolved in 1 liter of deionized water and kept at 50° C. for 10 hours. After the polyelectrolyte complex was dried in a blast dryer at 50° C. for 60 hours, it was ball-milled for 1 hour with a ball mill to obtain chitosan polyelectrolyte hemostatic powder. The hemostatic powder has good biocompatibility and antibacterial property, and exhibits excellent hemostatic function. The in vitro coagulation index measurement in this example shows that the in vitro coagulation index is <56%; the hemostatic effect test result of the rabbit ear artery bleeding model shows that the hemostatic time is <140 seconds.

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Abstract

The invention belongs to the field of biological medicinal material preparation, and relates to a preparation method of chitosan oligosaccharide-based polyelectrolyte styptic powder. The preparation method comprises the following steps of firstly dissolving chitosan oligosaccharide and an anionic polyelectrolyte or ampholytic polyelectrolyte having biocompatibility in deionized water; reducing the pH (Potential of Hydrogen) value of the solution, protonizing and positively charging amino groups of chitosan oligosaccharide molecules, and interacting with the anionic polyelectrolyte or ampholytic polyelectrolyte through coulomb force, thus forming a compound; drying and smashing the polyelectrolyte compound, thus obtaining the chitosan oligosaccharide-based polyelectrolyte styptic powder. The chitosan oligosaccharide-based polyelectrolyte styptic powder prepared by the preparation method disclosed by the invention has good biocompatibility and good antibacterial activity, and an excellent hemostatic function is reflected.

Description

technical field [0001] The invention belongs to the field of biomedical material preparation and relates to a preparation method of polyelectrolyte hemostatic powder based on chitosan oligosaccharide. Background technique [0002] Modern medical theory proves that wound healing is a continuous dynamic process of cell-to-cell, cell-to-cell matrix, and soluble media interactions. Wound repair is related to the interaction of local tissue nutrition, microenvironment, growth factor content, microcirculation, hypoxic conditions, infection and other factors, and medical dressings can play a key role. As a main theoretical basis of high-tech medical dressings, the "wet therapy" discovered by British scientists in 1962 believes that when the surface of the wound is maintained in a moist microenvironment, the migration of epithelial cells is significantly accelerated, and the healing speed of the wound is faster than that of wounds. Fast in dry conditions. [0003] Blood is an impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/24A61L15/32
Inventor 赵岩张文昌张劲松
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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