A rapid aggregation breeding method for multi-objective traits of high-quality broilers

A technology of aggregation breeding and multi-objective, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of low efficiency of trait selection, slow genotype homozygosity, etc., and achieve cost savings and Manpower, overcoming genotype homozygous slowness, and reducing the number of effects
CN106701935BActive Publication Date: 2020-01-07JIANGSU INST OF POULTRY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU INST OF POULTRY SCI
Publication Date
2020-01-07

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Abstract

The invention discloses a fast pyramiding breeding method of high-quality broiler multi-target traits. The fast pyramiding breeding method comprises the following steps: 1, extracting of genome DNA of broiler; 2, primer designing: designing a polymerase chain reaction amplification primer according to gene sequences of a growth differentiation factor 9, an estrogen receptor, a dopamine receptor 1, a dopamine receptor 2 and a vasoactive intestinal peptide receptor 2; 3, polymerase chain reaction; 4, single-nucleotide polymorphism reaction; 5, SSCP analysis on a target fragment, development by use of silver staining after gel electrophoresis to obtain an SSCP map of the DNA, selection of the growth differentiation factor 9, the estrogen receptor, the dopamine receptor 1, the dopamine receptor 2, the vasoactive intestinal peptide receptor 2 and individuals with genotypes of genes of QQ, GG, KK, TT and TT according to the banding pattern selection in 5 maps. The fast pyramiding breeding method disclosed by the invention is obvious in effect, the detection method is simple and fast and cannot be influenced by external environments, and an efficient and fast high-quality broiler pyramiding breeding new method is formed.
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Description

technical field

[0001] The invention relates to a high-quality broiler multi-target trait multi-gene aggregation breeding method, which belongs to poultry breeding methods, and specifically relates to multi-target trait assembly and aggregation, multi-gene aggregation trait screening, rapid fixation and homozygosity of target genotypes and other breeding methods. Background technique

[0002] The high-quality broiler breeding industry is a characteristic industry in my country, and the eternal goal of high-quality broiler breeding is "high efficiency and high quality". Compared with foreign fast and large white-feathered broilers, my country's high-quality broilers not only have higher requirements on the content of flavor substances and muscle quality, but also require breeders to have high reproductive performance and commercial chickens to have the characteristics of an appropriate growth rate. Breeding chickens can provide more commercial generation chickens and the appr...

Claims

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