A rapid aggregation breeding method for multi-objective traits of high-quality broilers

A technology of aggregation breeding and multi-objective, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of low efficiency of trait selection, slow genotype homozygosity, etc., and achieve cost savings and Manpower, overcoming genotype homozygous slowness, and reducing the number of effects

Active Publication Date: 2020-01-07
JIANGSU INST OF POULTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology overcomes the problems of slow genotype homozygosity and low efficiency of trait selection in conventional selective breeding of high-quality broilers, and gives full play to the role of biotechnology in poultry breeding, thus forming an efficient and fast new technology for high-quality broiler aggregation breeding

Method used

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  • A rapid aggregation breeding method for multi-objective traits of high-quality broilers
  • A rapid aggregation breeding method for multi-objective traits of high-quality broilers
  • A rapid aggregation breeding method for multi-objective traits of high-quality broilers

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Experimental program
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Embodiment

[0045] Example: Breeding of high-quality broiler early-maturing and high-breeding S3 lines.

[0046] The size and uniformity of the comb, the number of eggs laid at 43 weeks and the number of eggs laid at 66 weeks were selected as the traits for breeding.

[0047] Conventional selection: the selection methods of individual selection and family selection are adopted. The selection and retention rate of hens is 25-35%, and that of roosters is 5-7%. Closed breeding in each generation to avoid 1 / 4 inbreeding.

[0048] Breeding procedure:

[0049] 1. Wear the wing number according to the pedigree when hatching;

[0050] 2. Measure the comb size of the whole flock at the age of 10 weeks, sort according to the average weight of the family and the coefficient of variation, and eliminate the family with the average value of the comb that is too small and the coefficient of variation too large;

[0051] 3. In the selected families, sort all the individuals according to the comb size...

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Abstract

The invention discloses a fast pyramiding breeding method of high-quality broiler multi-target traits. The fast pyramiding breeding method comprises the following steps: 1, extracting of genome DNA of broiler; 2, primer designing: designing a polymerase chain reaction amplification primer according to gene sequences of a growth differentiation factor 9, an estrogen receptor, a dopamine receptor 1, a dopamine receptor 2 and a vasoactive intestinal peptide receptor 2; 3, polymerase chain reaction; 4, single-nucleotide polymorphism reaction; 5, SSCP analysis on a target fragment, development by use of silver staining after gel electrophoresis to obtain an SSCP map of the DNA, selection of the growth differentiation factor 9, the estrogen receptor, the dopamine receptor 1, the dopamine receptor 2, the vasoactive intestinal peptide receptor 2 and individuals with genotypes of genes of QQ, GG, KK, TT and TT according to the banding pattern selection in 5 maps. The fast pyramiding breeding method disclosed by the invention is obvious in effect, the detection method is simple and fast and cannot be influenced by external environments, and an efficient and fast high-quality broiler pyramiding breeding new method is formed.

Description

technical field [0001] The invention relates to a high-quality broiler multi-target trait multi-gene aggregation breeding method, which belongs to poultry breeding methods, and specifically relates to multi-target trait assembly and aggregation, multi-gene aggregation trait screening, rapid fixation and homozygosity of target genotypes and other breeding methods. Background technique [0002] The high-quality broiler breeding industry is a characteristic industry in my country, and the eternal goal of high-quality broiler breeding is "high efficiency and high quality". Compared with foreign fast and large white-feathered broilers, my country's high-quality broilers not only have higher requirements on the content of flavor substances and muscle quality, but also require breeders to have high reproductive performance and commercial chickens to have the characteristics of an appropriate growth rate. Breeding chickens can provide more commercial generation chickens and the appr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 赵振华黎寿丰李春苗黄华云王钱保吴兆林刘一帆赵东伟梁忠卢建李新
Owner JIANGSU INST OF POULTRY SCI
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