Citrus chachiensis hortorum polymethoxylated flavonoids effective part and application thereof for treating hyperlipidaemia

A technology of polymethoxylated flavonoids and effective parts, applied in the field of medicine, can solve problems such as hyperlipidemia without polymethoxylated flavonoids of tangerine peel, and achieve the effect of preventing or treating hyperlipidemia and reducing blood lipid levels

Inactive Publication Date: 2017-05-31
CHINA PHARM UNIV
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report that the effective parts of tangerine peel polymethoxylated flavonoids are used for the treatment of hyperlipidemia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Citrus chachiensis hortorum polymethoxylated flavonoids effective part and application thereof for treating hyperlipidaemia
  • Citrus chachiensis hortorum polymethoxylated flavonoids effective part and application thereof for treating hyperlipidaemia
  • Citrus chachiensis hortorum polymethoxylated flavonoids effective part and application thereof for treating hyperlipidaemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 The preparation of the effective fraction of polymethoxylated flavonoids from tangerine peel

[0041] Prepared by the following steps:

[0042] Step S1, extraction: crush the tangerine peel medicinal material into coarse particles, heat and reflux with 95% ethanol aqueous solution for extraction three times according to the solid-to-liquid ratio of 1:4, filter, combine the filtrates, and concentrate under reduced pressure to obtain leaching paste;

[0043] Step S2, impurity removal: after dissolving the extract obtained in step S1 with 25% ethanol aqueous solution by volume, filter the filtrate and apply it to D101 macroporous resin, and then elute with 43% ethanol aqueous solution 10 column volumes for impurity removal;

[0044] Step S3, elution: after removing impurities, elute with 95% ethanol aqueous solution for 8 column volumes, collect the eluate, and concentrate to obtain the effective fraction of polymethoxylated flavonoids of tangerine peel.

Embodiment 2

[0045] Example 2 The preparation of the effective fraction of tangerine peel polymethoxyflavone

[0046] Prepared by the following steps:

[0047] Step S1, extraction: crush the medicinal material of tangerine peel into coarse particles, heat and reflux with 90% ethanol aqueous solution with a volume percent content of 90% ethanol to extract three times according to the solid-to-liquid ratio, filter, combine the filtrates, and concentrate under reduced pressure to obtain leaching paste;

[0048] Step S2, impurity removal: after dissolving the extract obtained in step S1 with 20% ethanol aqueous solution by volume, filter the filtrate and apply it to D101 macroporous resin, and then elute with 40% ethanol aqueous solution 12 column volumes for impurity removal;

[0049] Step S3, elution: after removing impurities, elute with 90% ethanol aqueous solution for 12 column volumes, collect the eluate, and concentrate to obtain the effective fraction of polymethoxylated flavonoids o...

Embodiment 3

[0050] Example 3 The preparation of the effective fraction of tangerine peel polymethoxyflavone

[0051] Prepared by the following steps:

[0052] Step S1, extraction: crush the tangerine peel medicinal material into coarse particles, heat and reflux with absolute ethanol to extract three times according to the solid-to-liquid ratio of 1:4, filter, combine the filtrates, and concentrate under reduced pressure to obtain the extract;

[0053] Step S2, impurity removal: after dissolving the extract obtained in step S1 with 30% ethanol solution by volume, filter the filtrate and apply it to D101 macroporous resin, and then elute with 45% ethanol solution by volume 8 column volumes for impurity removal;

[0054] Step S3, elution: after removing impurities, elute with absolute ethanol for 6 column volumes, collect the eluate, and concentrate to obtain the effective fraction of polymethoxylated flavonoids of tangerine peel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a citrus chachiensis hortorum polymethoxylated flavonoids effective part and application thereof for treating hyperlipidaemia. The effective part is prepared from the following steps: step S1, extracting: grinding the citrus chachiensis hortorum, extracting by use of an alcohol-water solution, concentrating; step S2, removing impurities; sampling the concentrated solution obtained in the step S1 on macroporous resin, eluting and removing impurities by use of an ethanol water solution with the volume percent concentration of 40-45%, wherein the model of the macroporous resin is D101 type; and step S3, eluting: eluting the macroporous resin by use of the ethanol water solution with the volume percent concentration of 90% or more, collecting eluent, concentrating to obtain the citrus chachiensis hortorum polymethoxylated flavonoids effective part. The citrus chachiensis hortorum polymethoxylated flavonoids effective part provided by the invention can effectively lower the blood lipid level of hyperlipidaemia model mice, and is non-toxic, safe and effective, and can be used for preventing or treating hyperlipidaemia.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the preparation and application of effective parts of crude drugs, in particular to an effective part of polymethoxylated flavonoids of tangerine peel and its application in treating hyperlipidemia. Background technique [0002] Hyperlipidemia is a chronic disease in which lipid disorders lead to metabolic imbalances in the body, often manifested as high serum total cholesterol or triglyceride levels and low high-density lipoproteins. Hyperlipidemia is closely related to the formation of many chronic diseases such as fatty liver, diabetes, atherosclerosis, hypertension and cardiovascular diseases. In recent years, with the improvement of living standards and changes in diet structure, the number of people suffering from hyperlipidemia has been increasing. At present, the drugs for treating hyperlipidemia mainly include statins, niacin, fibrates, and bile acid integrators, among which stati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61P3/06G01N30/02
CPCA61K36/752A61K2236/333A61K2236/51A61K2236/53A61K2236/55G01N30/02
Inventor 刘鄂湖曾素玲李尚真俞可云窦莉莉李佩
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap