Culture medium applicable to dental pulp stem cell in vitro culture and preparation method thereof

A technique for in vitro culturing of dental pulp stem cells is applied to the culture medium for in vitro culturing of dental pulp stem cells and the field of preparation thereof.

Inactive Publication Date: 2017-05-31
叶宗耀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a serum-free medium for dental pulp stem cells has been developed, it is expensive

Method used

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  • Culture medium applicable to dental pulp stem cell in vitro culture and preparation method thereof
  • Culture medium applicable to dental pulp stem cell in vitro culture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, a kind of culture medium suitable for dental pulp stem cells cultured in vitro

[0031] The medium suitable for in vitro culture of dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: catalase 3mg / L, coenzyme Q10 0.2μM, linolenic acid 7μM, platelet-derived growth factor 1μg / L, cucurbitacin B0.2mg / L, lipoic acid 0.12mg / L, folic acid 12μM, carboxymethyl chitosan 0.5g / L, epidermal growth factor 8μg / L L, taurine 7mg / L and adherent material 22μg / L.

[0032] The basal medium is MEM medium.

[0033] The wall-adhering material is composed of epinectin and fibronectin in a weight ratio of 5:12.

[0034] Preparation:

[0035] S1 Add linolenic acid, platelet-derived growth factor, lipoic acid, folic acid, epidermal growth factor, taurine and adherent materials to the basal medium, stir for 15 minutes, add cucurbitacin B and carboxymethyl chitosan, and continue stirring 28 minutes, let...

Embodiment 2

[0037] Embodiment 2, a kind of culture medium suitable for dental pulp stem cells cultured in vitro

[0038] The medium suitable for in vitro culture of dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: catalase 5mg / L, coenzyme Q10 0.4μM, linolenic acid 9μM, platelet-derived growth factor 3μg / L, cucurbitacin B0.6mg / L, lipoic acid 0.15mg / L, folic acid 15μM, carboxymethyl chitosan 1g / L, epidermal growth factor 10μg / L , taurine 10mg / L and adherent material 30μg / L.

[0039] The basal medium is M199 medium.

[0040]The wall-adhering material is composed of epinectin and fibronectin in a weight ratio of 7:9.

[0041] Preparation:

[0042] S1 Add linolenic acid, platelet-derived growth factor, lipoic acid, folic acid, epidermal growth factor, taurine and adherent materials to the basal medium, stir for 22 minutes, add cucurbitacin B and carboxymethyl chitosan, and continue stirring 36 minutes, let s...

Embodiment 3

[0044] Embodiment 3, a kind of culture medium suitable for the in vitro culture of dental pulp stem cells

[0045] The medium suitable for in vitro culture of dental pulp stem cells includes basal medium and additives added in the basal medium, and the ingredients of the additives include: catalase 4mg / L, coenzyme Q10 0.3μM, linolenic acid 8μM, platelet-derived growth factor 2μg / L, cucurbitacin B0.5mg / L, lipoic acid 0.14mg / L, folic acid 13μM, carboxymethyl chitosan 0.8g / L, epidermal growth factor 9μg / L L, taurine 8mg / L and adherent material 27μg / L.

[0046] The basal medium is M199 medium.

[0047] The wall-adhering material is composed of epinectin and fibronectin in a weight ratio of 6:11.

[0048] Preparation:

[0049] S1 Add linolenic acid, platelet-derived growth factor, lipoic acid, folic acid, epidermal growth factor, taurine and adherent materials to the basal medium, stir for 18 minutes, add cucurbitacin B and carboxymethyl chitosan, and continue stirring 32 minut...

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Abstract

The invention belongs to the technical field of cell culture, and concretely relates to a culture medium applicable to dental pulp stem cell in vitro culture and a preparation method thereof. The culture medium applicable to dental pulp stem cell in vitro culture provided by the invention comprises a basal culture medium and an additive added in the basal culture medium, wherein the additive is prepared from the following components with final concentration: catalase with the final concentration of 3 to 5 mg / L, coenzyme Q10 with the final concentration of 0.2 to 0.4mu M, linolenic acid with the final concentration of 7 to 9mu M, platelet-derived growth factor with the final concentration of 1 to 3mu g / L, cucurbitacin B with the final concentration of 0.2 to 0.6mg / L, lipoic acid with the final concentration of 0.12 to 0.15mg / L, folic acid with the final concentration of 12 to 15mu M, carboxymethyl chitosan with the final concentration of 0.5 to 1g / L, epidermal growth factor with the final concentration of 8 to 10mu g / L, taurine with the final concentration of 7 to 10mg / L, and attachin with the final concentration of 22 to 30mu g / L. The culture medium provided by the invention is high in safety, lower in price, and beneficial for remarkably improving the proliferation speed of dental pulp stem cells.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a culture medium suitable for in vitro culture of dental pulp stem cells and a preparation method thereof. Background technique [0002] Dental defect and tooth loss due to various reasons have become the main diseases that endanger the oral cavity and even human health, and the incidence rate is increasing year by year. At present, various dental materials are mainly used to deal with tooth defect diseases. Although it can prevent the continued development of lesions to a certain extent, it cannot solve the fundamental problem. Although there are many solutions to tooth loss, the most effective and fundamental way is tooth regeneration. [0003] With the rapid development of tissue regenerative medicine, it brings hope for the effective treatment of dental defects and missing teeth. At present, many studies have used the interaction of odontogenic epithelium a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0664C12N2500/30C12N2500/32C12N2500/38C12N2500/90C12N2501/11C12N2501/135C12N2501/70C12N2501/71C12N2501/90
Inventor 叶宗耀
Owner 叶宗耀
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