Primer combination for detecting skin anti-aging capability genes, and detection method

A primer composition, anti-aging technology, applied in biochemical equipment and methods, recombinant DNA technology, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2017-05-31
上海东方杰玛基因生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The US media said that "new research shows that strengthening exercise, regular work and rest, and

Method used

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  • Primer combination for detecting skin anti-aging capability genes, and detection method
  • Primer combination for detecting skin anti-aging capability genes, and detection method
  • Primer combination for detecting skin anti-aging capability genes, and detection method

Examples

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Effect test

Embodiment 1

[0146] A method of using the primer composition of the present invention to detect skin anti-aging ability genes in this embodiment, the detection steps also include sample collection, DNA extraction, nucleic acid mass spectrometry-skin anti-aging ability genotype detection and genotype scoring , the specific steps are the same as those described above.

[0147] 1. Sample collection

[0148] The sample collection information is as follows, and the operation steps are the same as above.

[0149] sampling date initials Numbering sample type Number of samples 20160119 YX 160119005 oral exfoliated cells 4 oral swabs 20160221 LYF 160221001 oral exfoliated cells 4 oral swabs 20160221 ZX 160221002 oral exfoliated cells 4 oral swabs

[0150] 2. DNA extraction, the operation steps are the same as above.

[0151] The measured DNA concentrations of the three samples were as follows:

[0152] sample number 160119005 160...

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Abstract

The invention discloses a primer combination for detecting skin anti-aging capability genes. The skin anti-aging capability genes comprises collagen multiplication capability gene COL1A1, a skin moisture retaining and keeping capability gene AQP3, a radiation protection capability gene ASIP, ultraviolet damage repair capability genes ERCC2 and LOC105374069, a skin detoxifying capability gene GSTP1, a skin sensitivity gene IL6R and an estrogen level gene DIAPH2, and corresponding PCR primers and single-base extension reaction primers are designed aiming at the eight genes. The invention further discloses a method for performing gene detection by utilizing the primer combination. The method comprises the following steps: carrying out sample DNA extraction, PCR amplification and single-base extension and nucleic acid mass spectrometric analysis to obtain corresponding genotypes, and then carrying out analysis and scoring, thereby scientifically evaluating the difference of individual skin aging processes, providing scientific and precise recommendations for daily skin care and life habits of individuals, and also being helpful to reasonable selection of skincare products of individuals according to skin types.

Description

technical field [0001] The invention belongs to the technical field of molecular biology gene detection, in particular to a primer composition and a detection method for skin anti-aging ability gene detection. Background technique [0002] The US media said that "new research shows that strengthening exercise, regular work and rest, and using skin care products can help people stay young, but genetics may also predetermine the speed of aging." [0003] American scientist Tesman said: "Through the Human Genome Project, we can analyze hundreds of genetic changes that occur during aging. The skin ages continuously through eight different methods, each of which is controlled by its own genome." .whether you age slowly like Cliff Richard or get wrinkled like Keith Richards, it's all determined by your lifestyle and caused by these genes play a part". [0004] One of the most important factors Tesman and his team believe is hydration, the skin's method of collecting and retainin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/148C12Q2600/156
Inventor 李艳芳曾沃坦郭金海邹宇飞刘进稳
Owner 上海东方杰玛基因生物科技有限公司
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