Application of biomarker in diagnosis of Parkinson

A technology of biomarkers and uses, applied in UROD. , The application field of biomarkers in the diagnosis of Parkinson's can solve the problems of affecting, interfering with functional independence, interfering with daily life functions, etc., and achieve the effect of improving the quality of life

Inactive Publication Date: 2017-05-31
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cognitive impairment in PD-D patients obviously affects and interferes with daily life functions, while PD-MCI patients only show mild cognitive impairment, although there may be slight difficulties in performing complex functional tasks, which are not significant enough interference with functional independence

Method used

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  • Application of biomarker in diagnosis of Parkinson

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1 Screening for biomarkers related to Parkinson's

[0051] 1. Sample collection

[0052] 10 normal blood samples and blood samples from Parkinson's patients were collected, and all the above samples were obtained with the consent of the ethics committee.

[0053] Exclusion criteria for normal control group: (1) Parkinson's disease and family history of Parkinson's disease; (2) Alzheimer's disease. History of epilepsy, cerebrovascular disease and other neurological diseases; (3) previous history of mental illness. Such as schizophrenia, severe depression, mental retardation and mental disorders caused by physical diseases; (4) those who are illiterate, color blind, language barrier or unable to communicate due to other reasons.

[0054] Exclusion criteria for Parkinson's disease group: (1) People who are illiterate, color blind, language barrier or unable to communicate due to other reasons; (2) People with cognitive impairment or dementia caused by drug poison...

Embodiment 2

[0082] Example 2 QPCR sequencing to verify differential expression of UROD gene

[0083] 1. According to the detection results of high-throughput sequencing, the UROD gene was selected for large-sample QPCR verification. According to the sample collection method in Example 1, 80 cases of blood from Parkinson's patients and blood from normal people were selected.

[0084] 2. The RNA extraction steps are the same as in Example 1.

[0085] 3. Reverse transcription: use the reverse transcription kit from TIANGEN Company, the kit number is KR106. Specific steps are as follows:

[0086] To remove genomic DNA reaction, add 5×DNA Buffer 2.0μl, RNA 1μg to the test tube, add RNase-removing ddH 2 O Bring the total volume to 10 μl, heat in a water bath at 42°C for 3 minutes, then add 2.0 μL of 10×Fast RT Buffer, 1.0 μL of RTEnzyme Mix, 2.0 μL of FQ-RT Primer Mix, and remove RNase ddH 2 O 5.0 μL, mixed and added to the above test tube and mixed together, heated in a water bath at 42°C ...

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Abstract

The invention discloses application of a biomarker in diagnosis of Parkinson. Specifically, the biomarker is UROD, the invention firstly discovers that expression of UROD in a Parkinson patient is regulated downwards, showing that UROD expression level detection can become one index for the diagnosis of the Parkinson. The invention provides a product for realizing early diagnosis of Parkinson disease, early intervention is carried out by virtue of early discovery, and further survival quality and living quality of the patient can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a biomarker in diagnosing Parkinson's disease, specifically, the biomarker is UROD. Background technique [0002] Parkinson's disease (Parkinson's disease, PD), also known as Parkinson's paralysis, is a progressive degenerative disease of the central nervous system, which seriously affects people's quality of life. The main pathological feature of the disease is the substantia nigra pars compacta The degeneration and loss of dopaminergic neurons in the locus coeruleus, locus coeruleus, and raphe nucleus, and the formation of Lewy bodies (LB) in residual neurons. Epidemiological studies at home and abroad have shown that the incidence rate in people over 65 years old is 1-2 2%, 4-5% over the age of 85, and the total number of PD patients over the age of 55 has exceeded 1.7 million. With the natural aging of the social population, the number of PD patients is increasing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6896G01N2333/47G01N2800/2835
Inventor 孔屏雷平张释双李岱张永强赵静
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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