Extraction method and detection method for polysaccharide of lamiophlomis rotata

An extraction method, unique technology, applied in the field of extraction of unique polysaccharides, can solve the problems of no unique extraction method and its quality control method reported, and achieve high accuracy, good application prospects, and efficient extraction Effect

Inactive Publication Date: 2017-05-31
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the extraction method of the unique polysaccharide and its quality control method

Method used

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  • Extraction method and detection method for polysaccharide of lamiophlomis rotata
  • Extraction method and detection method for polysaccharide of lamiophlomis rotata
  • Extraction method and detection method for polysaccharide of lamiophlomis rotata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 The extraction method of unique polysaccharide of the present invention

[0034] 1. Extraction method

[0035] (1) Decolorization

[0036] Weigh 1g of Duyiwei powder for each portion, accurately weigh (n=2), wrap it with filter paper, place it in a Soxhlet extractor, add 50ml~100ml of 85% ethanol, and reflux until the extract is colorless (4h~ 8h), the residue is brown, and the ethanol is naturally evaporated for subsequent use to obtain a decolorized residue;

[0037] (2) Extraction

[0038] Take the decolorized residue, put it in a round-bottomed flask, add 30ml of distilled water, reflux in a water bath at 80°C for 60min, repeat 3 times, and combine the extracts.

[0039] 2. Detection of extraction rate

[0040] 2.1 Detection method

[0041] Take the extract, filter it with suction, and adjust the volume of the filtrate to 250ml, then measure 5ml of the solution in a 50ml volumetric flask, and add distilled water to volume up to the mark. Take 2ml ...

Embodiment 2

[0055] Example 2 Screening of the extraction method of the unique polysaccharide of the present invention

[0056] 1. Extraction times

[0057] 1. Extraction method

[0058] (1) Decolorization

[0059] Weigh 1g of Duyiwei powder for each portion, accurately weigh (n=2), wrap it with filter paper, place it in a Soxhlet extractor, add 50ml~100ml of 85% ethanol, and reflux until the extract is colorless (4h~ 8h), the residue is brown, and the ethanol is naturally evaporated for subsequent use to obtain a decolorized residue;

[0060] (2) Extraction

[0061]Take the decolorized residue, put it in a round-bottomed flask, add 30ml of distilled water, reflux in a water bath at 80°C for 60 minutes, do not repeat or repeat 1 time, repeat 2 times, repeat 3 times, repeat 4 times, combine the extracts, That's it.

[0062] 2. Detection of extraction rate

[0063] 2.1 Detection method

[0064] With embodiment 1.

[0065] 2.2 Test results

[0066] The test results are shown in Table...

Embodiment 3

[0137] Embodiment 3 The detection method of unique polysaccharide of the present invention

[0138] 1. Detection method

[0139] Preparation of reference solution: Accurately draw 0.2615mg / ml glucose standard solution 1.0ml, 1.5ml, 2.0ml, 2.5ml, 3.0ml, 3.5ml, 4.0ml, 4.5ml into a 25ml volumetric flask, add distilled water to scale, the reference solution was obtained.

[0140] The preparation of need testing solution: can be prepared according to embodiment 1 and embodiment 2 polysaccharide extraction method, and the present embodiment is prepared according to the method obtained in embodiment 1, specifically: each part takes by weighing 1g of unique powder, accurately weighed (n =2), wrapped with filter paper, placed in a Soxhlet extractor, added 50ml to 100ml of 85% ethanol, refluxed until the extract was colorless (4h to 8h), and the residue was brown, and the ethanol was naturally evaporated for subsequent use to obtain Residue after decolorization: take the residue after...

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Abstract

The invention discloses an extraction method for polysaccharide of lamiophlomis rotate. The extraction method comprises the following steps of taking lamiophlomis rotata powder, decoloring, adding water for reflux extraction, and collecting an extracting solution. The invention also discloses a method for detecting the content of the polysaccharide of the lamiophlomis rotate. According to the extraction method for the polysaccharide of the lamiophlomis rotate provided by the invention, the polysaccharide in a lamiophlomis rotate sample can be high-efficiently extracted, and the detection method established on the basis can be used for effectively and accurately detecting the content of the polysaccharide of the lamiophlomis rotate, is good in repeatability, high in accuracy, and capable of objectively reflecting the content of the polysaccharide of the lamiophlomis rotate sample, and has a favorable application prospect.

Description

technical field [0001] The invention relates to an extraction method and a detection method of unique polysaccharide. Background technique [0002] Duyiwei is derived from the whole herb of Lamiophlomis rotata, a plant of the Labiatae family. It is an important medicinal plant on the Qinghai-Tibet Plateau and a commonly used bulk Tibetan medicine. It is a first-class endangered Tibetan medicine species, mainly distributed in Gansu, Qinghai, Sichuan, Yunnan, and Tibet. etc. [2]. It is recorded in "Sibu Medical Code" and "Jingzhu Materia Medica" that this product has the functions of strengthening muscles and bones, drying yellow water, and relieving pain [3]. The 2010 edition of "Chinese Pharmacopoeia" records its functions and indications as: promoting blood circulation to stop bleeding, dispelling wind and relieving pain. For bruises, traumatic bleeding, rheumatic arthralgia, yellow water disease. It is reported in the literature that Duyiwei mainly contains iridoid glyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/31
CPCG01N1/28G01N21/31
Inventor 古锐钟世红
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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